Name: Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Monomer Protein (MHC-HM414)
Brand: KactusBio
SKU: MHC-HM414-100UG
Price: 371.8 USD
Availability: InStock

Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Monomer Protein (MHC-HM414)

Immobilized Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.

Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Monomer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.
Amino Acid Range: Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(B2M) and RMFPNAPYL peptide
Other Available Proteins:

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Catalog #MHC-HM414

Size

100UG

500UG

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Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Monomer Protein (MHC-HM414) Summary

Product Description	Recombinant Chimeric HLA-A02:01(mα3)&B2M&WT-1 (RMFPNAPYL) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr206(Human HLA-A02:01 α1&α2)&Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119 (B2M) and RMFPNAPYL peptide.
Product Background	The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A0201 of -the MHC Class I, B2M and RMFPNAPYL peptide of -the WT-1.
Product Category	Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs

Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Specifications

Protein	Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL)
Synonyms	MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33
MHC Form	Monomer
Allele	HLA-A*02:01
Accession	A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse H-2Ld α3)&P61769(B2M)&RMFPNAPYL
Species	Human&Mouse
Biotinylated	no
Amino Acid Range	Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(B2M) and RMFPNAPYL peptide
Molecular Weight	The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Chimeric HLA-A02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.
Product Tag	C-His-Avi
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Chimeric HLA-A02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA.
	Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Chimeric HLA-A*02:01 (mα3) &B2M&WT-1 (RMFPNAPYL) Monomer is greater than 95% as determined by SEC-HPLC.