Human&Mouse Chimeric WT-1 (HLA-A*02:01) Tetramer Protein (MHC-HM414T)
Catalog: MHC-HM414T
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| Product Description | Recombinant Human&Mouse Chimeric WT-1 (HLA-A*02:01) Tetramer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr206(Human HLA-A*02:01 α1&α2)&Asp207-Ala362(Mouse HLA-A*02:01 α3), Ile21-Met119 (B2M) and RMFPNAPYL peptide. |
| Product Category | Recombinant Protein |
| Target | WT-1 (HLA-A*02:01) Tetramer |
| Synonyms | MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33 |
| Gene/Protein ID | A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse HLA-A*02:01 α3)&P61769(B2M)&RMFPNAPYL |
| Species | Human&Mouse |
| Exact Sequence | Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Ala362(Mouse HLA-A*02:01 α3), Ile21-Met119(B2M)and RMFPNAPYL peptide |
| Molecular Weight | The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
| Activity | Immobilized Human&Mouse Chimeric WT-1 (HLA-A*02:01) Tetramer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 11.8ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Liquid |
| Shipping | Shipped with blue ice. |
| Formulation | Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label. |
| Stability And Storage | Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles. |
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Immobilized Human&Mouse Chimeric WT-1 (HLA-A*02:01) Tetramer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 11.8ng/ml determined by ELISA. |
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The purity of Human&Mouse Chimeric WT-1 (HLA-A*02:01) Tetramer is greater than 95% as determined by SEC-HPLC. |
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Human&Mouse Chimeric WT-1 (HLA-A*02:01) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%. |
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