Human&Mouse Chimeric WT-1 (HLA-A*02:01) Tetramer Protein (MHC-HM414T)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human&Mouse Chimeric WT-1 (HLA-A*02:01) Tetramer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 11.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Ala362(Mouse HLA-A*02:01 α3), Ile21-Met119(B2M)and RMFPNAPYL peptide

Catalog: MHC-HM414T

$1,125.00
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Product Description Recombinant Human&Mouse Chimeric WT-1 (HLA-A*02:01) Tetramer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr206(Human HLA-A*02:01 α1&α2)&Asp207-Ala362(Mouse HLA-A*02:01 α3), Ile21-Met119 (B2M) and RMFPNAPYL peptide.
Product Category Recombinant Protein
Target WT-1 (HLA-A*02:01) Tetramer
Synonyms MHC; WT1; RMF; AWT1; GUD; NPHS4GUD; WAGR; WIT-2; WT33
Gene/Protein ID A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse HLA-A*02:01 α3)&P61769(B2M)&RMFPNAPYL
Species Human&Mouse
Exact Sequence Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Ala362(Mouse HLA-A*02:01 α3), Ile21-Met119(B2M)and RMFPNAPYL peptide
Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Activity Immobilized Human&Mouse Chimeric WT-1 (HLA-A*02:01) Tetramer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 11.8ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
Immobilized Human&Mouse Chimeric WT-1 (HLA-A*02:01) Tetramer, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-WT-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 11.8ng/ml determined by ELISA.
The purity of Human&Mouse Chimeric WT-1 (HLA-A*02:01) Tetramer is greater than 95% as determined by SEC-HPLC.
Human&Mouse Chimeric WT-1 (HLA-A*02:01) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

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