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Human MSLN/Mesothelin Protein, Ultra Low Endotoxin (MSL-HM280-UL) Summary
Product Description |
Recombinant Human MSLN/Mesothelin Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Glu296-Gly580. |
Product Background |
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets | ADC targets |
Human MSLN/Mesothelin Protein, Ultra Low Endotoxin (MSL-HM280-UL) Specifications/Details
Protein |
MSLN/Mesothelin |
Synonyms |
MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu296-Gly580 |
Molecular Weight |
The protein has a predicted MW of 59 kDa. Due to glycosylation, the protein migrates to 68-78 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CA125, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human MSLN, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA (QC Test). |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human CA125, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human MSLN, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA (QC Test). |
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Immobilized Human MSLN at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-MSLN Antibody, hFc Tag with the EC50 of 44.2ng/ml determined by ELISA. |
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Human MSLN on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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Loaded Human CA125, His-Avi Tag on Anti-His-Biosensor can bind Human MSLN, hFc Tag with an affinity constant of 31.00 nM as determined in BLI assay . |
Other Available Proteins:
Finding available proteins...
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