Product Description |
Recombinant Human MUC18/CD146 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val24-Gly559. |
Product Background |
METCAM/MUC18 is an integral membrane cell adhesion molecule (CAM) in the Ig-like gene super-family. It can carry out common functions of CAMs which is to perform intercellular interactions and interaction of cell with extracellular matrix in tumor microenvironment, to interact with various signaling pathways and to regulate general behaviors of cells.The above positive correlation is fortuitous; actually METCAM/MUC18 acts as a tumor and metastasis suppressor for the ovarian carcinoma cells. |
Product Category |
Recombinant Protein |
Target |
MUC18/CD146 |
Synonyms |
Gicerin; CD146; MCAM; MUC18 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Val24-Gly559 |
Molecular Weight |
The protein has a predicted MW of 60.8 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human MUC18, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MUC18, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human MUC18, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MUC18, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA (QC Test). |
|
|
Human MUC18 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human MUC18 is greater than 95% as determined by SEC-HPLC. |
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