Human MUC18/CD146 Protein (MUC-HM118)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human MUC18, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MUC18, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Val24-Gly559

Catalog: MUC-HM118

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Product Description	Recombinant Human MUC18/CD146 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val24-Gly559.
Product Background	METCAM/MUC18 is an integral membrane cell adhesion molecule (CAM) in the Ig-like gene super-family. It can carry out common functions of CAMs which is to perform intercellular interactions and interaction of cell with extracellular matrix in tumor microenvironment, to interact with various signaling pathways and to regulate general behaviors of cells.The above positive correlation is fortuitous; actually METCAM/MUC18 acts as a tumor and metastasis suppressor for the ovarian carcinoma cells.
Product Category	Recombinant Protein

Target	MUC18/CD146
Synonyms	Gicerin; CD146; MCAM; MUC18
Gene/Protein ID	P43121
Species	Human
Exact Sequence	Val24-Gly559
Molecular Weight	The protein has a predicted MW of 60.8 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human MUC18, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MUC18, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human MUC18, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MUC18, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA.
	The purity of Human MUC18/CD146 is greater than 95% as determined by SEC-HPLC.
	Human MUC18/CD146 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.