• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Mucin-1, hFc Tag at 0.5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Anti-Mucin-1 Antibody, hFc Avi Tag with the EC50 of 23.1ng/ml determined by ELISA.
  • Amino Acid Range: Ala961-Ser1152
Catalog #MUC-HM201-UL
Price
Regular price $36300
Regular price Sale price $36300
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Size
Avantor VWR Fisher Scientific

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Human Mucin-1/MUC-1 Protein, Ultra Low Endotoxin (MUC-HM201-UL) Summary

Product Description

 Recombinant Human Mucin-1/MUC-1 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Ala961-Ser1152.

Product Background

 Mucin 1 (MUC1) is a transmembrane protein mainly expressed by epithelial and hematopoietic cells. It has a receptor-like structure, which can sense the external environment and activate intracellular signal transduction pathways through its cytoplasmic domain.

Human Mucin-1/MUC-1 Protein, Ultra Low Endotoxin (MUC-HM201-UL) Specifications/Details

Protein
 Mucin-1/MUC-1
Synonyms
 MUC1; MUC-1; Mucin-1; Mucin1; CA 15-3; Episialin; H23AG; KL-6; PEMT; PUM; PEM; EMA; CD227;;CA15-3; KL6
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala961-Ser1152
Molecular Weight
 The protein has a predicted MW of 46.61 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Mucin-1, hFc Tag at 0.5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Anti-Mucin-1 Antibody, hFc Avi Tag with the EC50 of 23.1ng/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Mucin-1, hFc Tag at 0.5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Anti-Mucin-1 Antibody, hFc Avi Tag with the EC50 of 23.1ng/ml determined by ELISA. Immobilized Human Mucin-1, hFc Tag at 0.5ug/ml(100ul/well) on the plate. Dose response curve for Biotinylated Anti-Mucin-1 Antibody, hFc Avi Tag with the EC50 of 23.1ng/ml determined by ELISA.
Human Mucin-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Mucin-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Mucin-1 is greater than 95% as determined by SEC-HPLC. The purity of Human Mucin-1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...