Human Mucin-13 Protein, Ultra Low Endotoxin (MUC-HM113-UL)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human Mucin-13, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Mucin-13 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
  • Amino Acid Range: Thr19-Leu421
Catalog #MUC-HM113-UL
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Size
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Human Mucin-13 Protein, Ultra Low Endotoxin (MUC-HM113-UL) Summary

Product Description

 Recombinant Human Mucin-13 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Thr19-Leu421.

Product Background

 Mucin 13 (MUC13) is a high-molecular-weight transmembrane glycoprotein that is frequently and aberrantly expressed in a variety of epithelial carcinomas, including gastric, colorectal, and ovarian cancers. MUC13 exhibits the characteristics suitable as an early marker for cancer screening and presents a promising target for antibody-guided targeted therapy.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human Mucin-13 Protein, Ultra Low Endotoxin (MUC-HM113-UL) Specifications/Details

Protein
 Mucin-13
Synonyms
 Mucin-13; MUC13; DRCC1; RECC;
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Thr19-Leu421
Molecular Weight
 The protein has a predicted MW of 44.19 kDa. Due to glycosylation, the protein migrates to 75-110 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Mucin-13, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Mucin-13 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human Mucin-13, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Mucin-13 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA. Immobilized Human Mucin-13, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Mucin-13 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
Human Mucin-13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Mucin-13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Anti-Mucin-13 Antibody, hFc Tag on Streptavidin-Biosensor can bind Human Mucin-13, His Tag with an affinity constant of 8.81 nM as determined in BLI assay. Loaded Anti-Mucin-13 Antibody, hFc Tag on Streptavidin-Biosensor can bind Human Mucin-13, His Tag with an affinity constant of 8.81 nM as determined in BLI assay.
The purity of Human Mucin-13 is greater than 90% as determined by SEC-HPLC. The purity of Human Mucin-13 is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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