Product Description |
Recombinant Human Nectin-1/PVRL1/CD111 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Gln31-Thr334. |
Product Background |
Nectins are a small family of Ca -independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions. They are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins.Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between Nectin-1 and Nectin-3 and between Nectin-1 and Nectin-4. Has some neurite outgrowth-promoting activity. |
Product Category |
Recombinant Protein |
Target |
Nectin-1/PVRL1/CD111 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln31-Thr334 |
Molecular Weight |
The protein has a predicted MW of 59.89 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Nectin-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human Nectin-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 14.7ng/ml determined by ELISA (QC Test). |
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Immobilized Human Nectin-4, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 0.27ug/ml determined by ELISA. |
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Human Nectin-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Nectin-1 is greater than 95% as determined by SEC-HPLC. |
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