Human Nectin-1/PVRL1/CD111 Protein (NEC-HM401)
Catalog: NEC-HM401
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| Product Description | Recombinant Human Nectin-1/PVRL1/CD111 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal.It contains Gln31-Thr334. |
| Product Category | Recombinant Protein |
| Target | Nectin-1/PVRL1/CD111 |
| Synonyms | CD111; ED4; HIgRPVRR; HVEC; MGC142031; Nectin1; PRR; PRR1; PRR1MGC16207; PVRL1; PVRR1; SK-12; PVRL1; CLPED1; HIgR; OFC7; PVRR |
| Gene/Protein ID | Q15223 |
| Species | Human |
| Exact Sequence | Gln31-Thr334 |
| Molecular Weight | The protein has a predicted MW of 36.9 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 28.13 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Human Nectin-1, His Tag with an affinity constant of 28.13 nM as determined in SPR assay (Biacore T200). |
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Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.204 μM as determined in SPR assay (Biacore T200). |
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The purity of Human Nectin-1 is greater than 95% as determined by SEC-HPLC. |
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Human Nectin-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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