Human Nectin-2/CD112 Protein (NEC-HM002)
  • Express System: HEK293
  • Product Tag: No Tag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Nectin-2, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.19ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln32-Leu360
Catalog #NEC-HM002
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Product Description
 Recombinant Human Nectin-2/CD112 Protein is expressed from HEK293 without tag.It contains Gln32-Leu360.
Product Background
 Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.
Product Category
 Recombinant Protein
Target
 Nectin-2/CD112
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gln32-Leu360
Molecular Weight
 The protein has a predicted MW of 35.54 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Nectin-2, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.19ug/ml determined by ELISA. See testing image for detail.
Product Tag
 No Tag
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Nectin-2, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.19ug/ml determined by ELISA.
Human Nectin-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Nectin-2 is greater than 95% as determined by SEC-HPLC.

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