Human Nectin-2/CD112 Protein (NEC-HM202)
Human Nectin-2/CD112 Protein (NEC-HM202)
Human Nectin-2/CD112 Protein (NEC-HM202)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human PVRIG, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-2, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA. The affinity constant of 6.27 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln32-Leu360
Catalog #NEC-HM202
Regular price $264.00
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Human Nectin-2/CD112 Protein (NEC-HM202) Summary

Product Description

Recombinant Human Nectin-2/CD112 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln32-Leu360.

Product Background

Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Human Nectin-2/CD112 Protein (NEC-HM202) Specifications/Details

Protein
Nectin-2/CD112
Synonyms
CD112; MPH; Nectin2; PRR2; PVRL2; HVEB; PVRR2
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln32-Leu360
Molecular Weight
The protein has a predicted MW of 62.3 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human PVRIG, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-2, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA. The affinity constant of 6.27 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human Nectin-2, hFc Tag captured on CM5 Chip via Protein A can bind Human PVRIG, His Tag with an affinity constant of 6.27 uM as determined in SPR assay (Biacore T200). Human Nectin-2, hFc Tag captured on CM5 Chip via Protein A can bind Human PVRIG, His Tag with an affinity constant of 6.27 uM as determined in SPR assay (Biacore T200).
Immobilized Human PVRIG, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-2, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA. Immobilized Human PVRIG, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-2, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA.
Human Nectin-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Nectin-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Nectin-2 is greater than 95% as determined by SEC-HPLC. The purity of Human Nectin-2 is greater than 95% as determined by SEC-HPLC.
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