Human Nectin-2/CD112 Protein (NEC-HM202)
Catalog: NEC-HM202
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| Product Description | Recombinant Human Nectin-2/CD112 Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains Gln32-Leu360. |
| Product Category | Recombinant Protein |
| Target | Nectin-2/CD112 |
| Synonyms | CD112; MPH; Nectin2; PRR2; PVRL2; HVEB; PVRR2 |
| Gene/Protein ID | Q92692-2 |
| Species | Human |
| Exact Sequence | Gln32-Leu360 |
| Molecular Weight | The protein has a predicted MW of 62.3 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human PVRIG, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human Nectin-2, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human PVRIG, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human Nectin-2, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA. |
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The purity of Human Nectin-2 is greater than 95% as determined by SEC-HPLC. |
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Human Nectin-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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