Human Nectin-2/CD112 Protein (NEC-HM202)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human PVRIG, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human Nectin-2, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln32-Leu360

Catalog: NEC-HM202

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Product Description Recombinant Human Nectin-2/CD112 Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains Gln32-Leu360.
Product Category Recombinant Protein
Target Nectin-2/CD112
Synonyms CD112; MPH; Nectin2; PRR2; PVRL2; HVEB; PVRR2
Gene/Protein ID Q92692-2
Species Human
Exact Sequence Gln32-Leu360
Molecular Weight The protein has a predicted MW of 62.3 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human PVRIG, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human Nectin-2, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human PVRIG, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human Nectin-2, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA.
The purity of Human Nectin-2 is greater than 95% as determined by SEC-HPLC.
Human Nectin-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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