Name: Human Nectin-2/CD112 Protein (NEC-HM202)
Brand: KactusBio
SKU: NEC-HM202-100UG
Price: 264.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PVRIG, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-2, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA. The affinity constant of 6.27 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln32-Leu360

Catalog #NEC-HM202

Size

100UG

500UG

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Product Description	Recombinant Human Nectin-2/CD112 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln32-Leu360.
Product Background	Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein	Nectin-2/CD112
Synonyms	CD112; MPH; Nectin2; PRR2; PVRL2; HVEB; PVRR2
Accession	Q92692-2
Species	Human
Biotinylated	no
Amino Acid Range	Gln32-Leu360
Molecular Weight	The protein has a predicted MW of 62.3 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human PVRIG, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-2, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA. The affinity constant of 6.27 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human Nectin-2, hFc Tag captured on CM5 Chip via Protein A can bind Human PVRIG, His Tag with an affinity constant of 6.27 uM as determined in SPR assay (Biacore T200).
	Immobilized Human PVRIG, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-2, hFc Tag with the EC50 of 24.1ng/ml determined by ELISA.
	Human Nectin-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Nectin-2 is greater than 95% as determined by SEC-HPLC.