Human Nectin-2/CD112 Protein (NEC-HM402)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Nectin-2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.80μg/ml determined by ELISA. The affinity constant of 1.84 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln32-Leu360

Catalog: NEC-HM402

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Product Description Recombinant Human Nectin-2 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gln32-Leu360.
Product Category Recombinant Protein
Target Nectin-2/CD112
Synonyms CD112; MPH; Nectin2; PRR2; PVRL2; HVEB; PVRR2
Gene/Protein ID Q92692
Species Human
Exact Sequence Gln32-Leu360
Molecular Weight The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Nectin-2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.80μg/ml determined by ELISA. The affinity constant of 1.84 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-2, His-Avi Tag with an affinity constant of 1.84 μM as determined in SPR assay (Biacore T200).
Human PVRIG, mFc Tag captured on CM5 Chip via anti-mouse antibody can bind Human Nectin-2, His Tag with an affinity constant of 0.349 μM as determined in SPR assay (Biacore T200).
Immobilized Human Nectin-2, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.80μg/ml determined by ELISA.
The purity of Human Nectin-2 is greater than 95% as determined by SEC-HPLC.
Human Nectin-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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