Human Nectin-2/CD112 Protein (NEC-HM402)
Catalog: NEC-HM402
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| Product Description | Recombinant Human Nectin-2 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gln32-Leu360. |
| Product Category | Recombinant Protein |
| Target | Nectin-2/CD112 |
| Synonyms | CD112; MPH; Nectin2; PRR2; PVRL2; HVEB; PVRR2 |
| Gene/Protein ID | Q92692 |
| Species | Human |
| Exact Sequence | Gln32-Leu360 |
| Molecular Weight | The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human Nectin-2, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.15μg/ml determined by ELISA (QC Test). The affinity constant of 1.84 μM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-2, His-Avi Tag with an affinity constant of 1.84 μM as determined in SPR assay (Biacore T200). |
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Human PVRIG, mFc Tag captured on CM5 Chip via anti-mouse antibody can bind Human Nectin-2, His Tag with an affinity constant of 0.349 μM as determined in SPR assay (Biacore T200). |
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Immobilized Human Nectin-2, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.15μg/ml determined by ELISA (QC Test). |
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The purity of Human Nectin-2 is greater than 95% as determined by SEC-HPLC. |
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Human Nectin-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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