Product Description |
Recombinant Human Nectin-2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln32-Leu360. |
Product Background |
Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC. |
Product Category |
Recombinant Protein |
Protein |
Nectin-2/CD112 |
Synonyms |
CD112; MPH; Nectin2; PRR2; PVRL2; HVEB; PVRR2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln32-Leu360 |
Molecular Weight |
The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 92.1ng/ml determined by ELISA (QC Test). The affinity constant of 1.84 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-2, His-Avi Tag with an affinity constant of 1.84 uM as determined in SPR assay (Biacore T200). |
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Human PVRIG, mFc Tag captured on CM5 Chip via Protein A can bind Human Nectin-2, His Tag with an affinity constant of 87.49 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 92.1ng/ml determined by ELISA (QC Test). |
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Human Nectin-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Nectin-2 is greater than 95% as determined by SEC-HPLC. |
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