Human Nectin-2/CD112 Protein (NEC-HM402)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test). The affinity constant of 1.84 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gln32-Leu360

Catalog: NEC-HM402

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Product Description	Recombinant Human Nectin-2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln32-Leu360.
Product Background	Nectin-2 is an adhesion molecule that has been reported to play a role in tumor growth, metastasis and tumor angiogenesis. Nectin-2 expression in ovarian cancer may support tumor cell adhesion, leading to growth and lymph node metastasis. Effect of VEGF on Nectin-2 expression as well as permeability was investigated in HUVEC.
Product Category	Recombinant Protein

Target	Nectin-2/CD112
Synonyms	CD112; MPH; Nectin2; PRR2; PVRL2; HVEB; PVRR2
Gene/Protein ID	Q92692-2
Species	Human
Exact Sequence	Gln32-Leu360
Molecular Weight	The protein has a predicted MW of 38.4 kDa. Due to glycosylation, the protein migrates to 45-52 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test). The affinity constant of 1.84 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human TIGIT, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-2, His-Avi Tag with an affinity constant of 1.84 uM as determined in SPR assay (Biacore T200).
	Human PVRIG, mFc Tag captured on CM5 Chip via anti-mouse antibody can bind Human Nectin-2, His Tag with an affinity constant of 0.349 uM as determined in SPR assay (Biacore T200).
	Immobilized Human Nectin-2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human PVRIG, mFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test).
	The purity of Human Nectin-2 is greater than 95% as determined by SEC-HPLC.
	Human Nectin-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.