Human Nectin-3/CD113 Protein (NEC-HM203)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Nectin-3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 0.24ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly58-Asp400

Catalog: NEC-HM203

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Product Description Recombinant Human Nectin-3/CD113 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Gly58-Asp400.

Product Background

The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein. Its precursor is 549 amino acids (aa) in length. It contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa (aa 405-425), and a cytoplasmic region of 124 amino acids.

Product Category

Recombinant Protein

Target

Nectin-3/CD113
Accession

Species

Human

Exact Sequence

Gly58-Asp400
Molecular Weight The protein has a predicted MW of 63.63 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human Nectin-3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 0.24ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human Nectin-3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 0.24ug/ml determined by ELISA.
The purity of Human Nectin-3 is greater than 95% as determined by SEC-HPLC.
Human Nectin-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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