Human Nectin-3/CD113 Protein (NEC-HM203)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Nectin-3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 0.24ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly58-Asp400
Catalog #NEC-HM203
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Product Description
 Recombinant Human Nectin-3/CD113 Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Gly58-Asp400.
Product Background
 The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein. Its precursor is 549 amino acids (aa) in length. It contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa (aa 405-425), and a cytoplasmic region of 124 amino acids.
Product Category
 Recombinant Protein
Target
 Nectin-3/CD113
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly58-Asp400
Molecular Weight
 The protein has a predicted MW of 63.63 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Nectin-3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 0.24ug/ml determined by ELISA. See testing image for detail.
Product Tag
 C-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Nectin-3, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 0.24ug/ml determined by ELISA.
Human Nectin-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Nectin-3 is greater than 95% as determined by SEC-HPLC.
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