Human Nectin-3/CD113 Protein (NEC-HM203)

Immobilized Human Nectin-3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag (Cat. NEC-HM401) with the EC50 of 77.8ng/ml determined by ELISA.

Human Nectin-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human Nectin-3 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Nectin-3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag (Cat. NEC-HM401) with the EC50 of 77.8ng/ml determined by ELISA.
Amino Acid Range: Gly58-Asp400

Catalog #NEC-HM203

Price

$264⁰⁰

Size

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Human Nectin-3/CD113 Protein (NEC-HM203) Summary

Product Description

Recombinant Human Nectin-3/CD113 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Gly58-Asp400.

Product Background

The Nectin family has at least four members (Nectin-1‑4), all of which show alternate splicing, a transmembrane (TM) region (except for Nectin-1 gamma), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such, have been alternatively-named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus. Nectin-3 (also named PRR3, CD113, and PVRL3) is an 83 kDa, type I TM glycoprotein. Its precursor is 549 amino acids (aa) in length. It contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa (aa 405-425), and a cytoplasmic region of 124 amino acids.

Human Nectin-3/CD113 Protein (NEC-HM203) Specifications/Details

Protein	Nectin-3/CD113
Synonyms	CD113; CDw113; FLJ90624; PPR3; PVRL3; PVRR3; PRR3
Accession	Q9NQS3
Species	Human
Biotinylated	no
Amino Acid Range	Gly58-Asp400
Molecular Weight	The protein has a predicted MW of 63.63 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Nectin-3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag (Cat. NEC-HM401) with the EC50 of 77.8ng/ml determined by ELISA.
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human Nectin-3, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag (Cat. NEC-HM401) with the EC50 of 77.8ng/ml determined by ELISA.
	Human Nectin-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Nectin-3 is greater than 95% as determined by SEC-HPLC.