Human Nectin-3/CD113 Protein (NEC-HM403)
Catalog: NEC-HM403
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| Product Description | Recombinant Human Nectin-3/CD113 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gly58-Asp400. |
| Product Category | Recombinant Protein |
| Target | Nectin-3/CD113 |
| Synonyms | CD113; CDw113; FLJ90624; PPR3; PVRL3; PVRR3; PRR3 |
| Gene/Protein ID | Q9NQS3 |
| Species | Human |
| Exact Sequence | Gly58-Asp400 |
| Molecular Weight | The protein has a predicted MW of 40.6 kDa. Due to glycosylation, the protein migrates to 68-82 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 44μM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in 20mM PB, 250mM NaCl (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human TIGIT captured on Protein A chip, can bind Human Nectin-3, His Tag with an affinity constant of 44μM as determined in a SPR assay (Biacore T200). |
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Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.204 μM as determined in SPR assay (Biacore T200). |
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The purity of Human Nectin-3 is greater than 95% as determined by SEC-HPLC. |
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Human Nectin-3 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. |
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