Human NGAL/Lipocalin-2 Protein (NGL-HM102)
Human NGAL/Lipocalin-2 Protein (NGL-HM102)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human NGAL, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-NGAL Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gln21-Gly198
Catalog #NGL-HM102
Regular price $203.00
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Product Description
Recombinant Human NGAL/Lipocalin-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln21-Gly198.
Product Background
Acute kidney injury (AKI) is one of the most common complications of various serious conditions, and early diagnosis is therefore critical for the treatment of AKI. Recent evidence demonstrates that neutrophil gelatinase- associated lipocalin (NGAL) is closely associated with AKI. Several experimental and clinical studies have shown that the expression of urine and serum NGAL increases significantly in AKI. NGAL shows potential to be a new effective early biochemical marker of AKI. Further studies are needed to confirm the significant advantages of NGAL in the diagnosis of early AKI and its value in clinical applications.
Product Category
Recombinant Protein
Target
NGAL/Lipocalin-2
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln21-Gly198
Molecular Weight
The protein has a predicted MW of 21.64 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human NGAL, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-NGAL Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human NGAL, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-NGAL Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA (QC Test).
Human NGAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human NGAL is greater than 95% as determined by SEC-HPLC.
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