Human NKG2A/CD159a Protein (NKG-HM210)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human NKG2A, hFc Tag at 2ug/ml (100ul/Well) on Fc Antibody (2ug/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 37.1ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Arg100-Ala219

Catalog: NKG-HM210

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Product Description	Recombinant Human NKG2A/CD159a Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Arg100-Ala219.
Product Background	NKG2, also known as NKG2A(CD159A), is a member of the killer cell lectin-like receptor family. NKG2A marks a unique immune effector subset preferentially co-expressing the tissue-resident CD103 molecule, but not immune checkpoint inhibitors.It is expressed only in NK-cells, but not in T-cells or B-cells.
Product Category	Recombinant Protein

Target	NKG2A/CD159a
Synonyms	NKG2A; NKG2-A; CD94; KLRD1; KP43; KLRC1; CD159A
Gene/Protein ID	P26715-1
Species	Human
Exact Sequence	Arg100-Ala219
Molecular Weight	The protein has a predicted MW of 41.1 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human NKG2A, hFc Tag at 2ug/ml (100ul/Well) on Fc Antibody (2ug/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 37.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human NKG2A, hFc Tag at 2ug/ml (100ul/Well) on Fc Antibody (2ug/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 37.1ng/ml determined by ELISA.
	The purity of Human NKG2A is greater than 95% as determined by SEC-HPLC.
	Human NKG2A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.