ELISA binding curve of human NKG2A hFc-tag protein showing OD450 response to biotinylated anti-NKG2A antibody concentrations.
Human NKG2A/CD159a Protein (NKG-HM210)
Human NKG2A/CD159a Protein (NKG-HM210)
  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human NKG2A, hFc Tag at 5ug/ml (100ul/Well) on Fc Antibody (2ug/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Arg100-Ala219
Catalog #NKG-HM210
Regular price $346.50
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Human NKG2A/CD159a Protein (NKG-HM210) Summary

Product Description

Recombinant Human NKG2A/CD159a Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Arg100-Ala219.

Product Background

NKG2, also known as NKG2A(CD159A), is a member of the killer cell lectin-like receptor family. NKG2A marks a unique immune effector subset preferentially co-expressing the tissue-resident CD103 molecule, but not immune checkpoint inhibitors.It is expressed only in NK-cells, but not in T-cells or B-cells.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human NKG2A/CD159a Protein (NKG-HM210) Specifications/Details

Protein
NKG2A/CD159a
Synonyms
NKG2A; NKG2-A; CD94; KLRD1; KP43; KLRC1; CD159A
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Arg100-Ala219
Molecular Weight
The protein has a predicted MW of 41.1 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human NKG2A, hFc Tag at 5ug/ml (100ul/Well) on Fc Antibody (2ug/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

N-hFc
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human NKG2A, hFc Tag at 5ug/ml (100ul/Well) on Fc Antibody (2ug/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA (QC Test). Immobilized Human NKG2A, hFc Tag at 5ug/ml (100ul/Well) on Fc Antibody (2ug/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 27.0ng/ml determined by ELISA (QC Test).
Human NKG2A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human NKG2A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human NKG2A is greater than 95% as determined by SEC-HPLC. The purity of Human NKG2A is greater than 95% as determined by SEC-HPLC.
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