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Human NKG2A&CD94 Protein (NKC-HM295) Summary
Product Description |
Recombinant Human NKG2A&CD94 Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus of NKG2A and Flag tag at the N-Terminus of CD94.It contains Arg100-Leu233(NKG2A) and Ser34-Ile179(CD94). |
Product Background |
The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human NKG2A&CD94 Protein (NKC-HM295) Specifications/Details
Protein |
NKG2A&CD94 |
Synonyms |
CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94) |
Molecular Weight |
The protein has a predicted MW of 41.1 kDa (NKG2A)&17.9 kDa (CD94). Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human NKG2A&CD94, hFc Tag at 2ug/ml (100ul/Well) on Fc Antibody (2ug/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 14.1ng/ml determined by ELISA. |
Product Tag |
N-hFc (IgG1), N-Flag |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Immobilized Human NKG2A&CD94, hFc Tag at 2ug/ml (100ul/Well) on Fc Antibody (2ug/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 14.1ng/ml determined by ELISA. |
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Human NKG2A&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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