Human NKG2A&CD94 Protein (NKC-HM295)

  • Express System: HEK293
  • Product Tag: N-hFc, N-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human NKG2A&CD94, hFc Tag at 2ug/ml (100ul/Well) on Fc Antibody (2ug/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 40.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)

Catalog: NKC-HM295

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Product Description Recombinant Human NKG2A&CD94 Protein is expressed from HEK293 with hFc tag and Flag tag at the N-Terminus.It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94).

Product Background

The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.

Product Category

Recombinant Protein

Target

NKG2A&CD94
Synonyms CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94
Accession

Species

Human

Amino Acid Range

Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)
Molecular Weight The protein has a predicted MW of 41.1 kDa (NKG2A)&17.9 kDa (CD94). Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human NKG2A&CD94, hFc Tag at 2ug/ml (100ul/Well) on Fc Antibody (2ug/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 40.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-hFc, N-Flag

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human NKG2A&CD94, hFc Tag at 2ug/ml (100ul/Well) on Fc Antibody (2ug/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 40.8ng/ml determined by ELISA.
The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.
Human NKG2A&CD94 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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