Human NKG2A&CD94 Protein (NKC-HM295)
Catalog: NKC-HM295
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| Product Description | Recombinant Human NKG2A&CD94 Protein is expressed from Expi293 with hFc tag and Flag tag at the N-terminal.It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94). |
| Product Category | Recombinant Protein |
| Target | NKG2A&CD94 |
| Synonyms | CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94 |
| Gene/Protein ID | P26715-1(NKG2A)&Q13241-1(CD94) |
| Species | Human |
| Exact Sequence | Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94) |
| Molecular Weight | The protein has a predicted MW of 41.1 kDa (NKG2A)&17.9 kDa (CD94). Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human NKG2A&CD94, hFc Tag at 2μg/ml (100μl/Well) on Fc Antibody (2μg/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 40.8ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | N-hFc-Flag |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human NKG2A&CD94, hFc Tag at 2μg/ml (100μl/Well) on Fc Antibody (2μg/ml) precoated plate. Dose response curve for Biotinylated Anti-NKG2A Antibody, hFc Tag with the EC50 of 40.8ng/ml determined by ELISA. |
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The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC. |
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Human NKG2A&CD94 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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