Human NKG2A&CD94 Protein (NKC-HM394)

  • Express System: Expi293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human NKG2A&CD94, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA. The affinity constant of 7.90 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)

Catalog: NKC-HM394

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Product Description Recombinant Human NKG2A&CD94 Protein is expressed from Expi293 with mFc tag at the C-terminal.It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94).
Product Category Recombinant Protein
Target NKG2A&CD94
Synonyms CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94
Gene/Protein ID P26715-1(NKG2A)&&Q13241-1(CD94)
Species Human
Exact Sequence Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)
Molecular Weight The protein has a predicted MW of 56.9 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human NKG2A&CD94, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA. The affinity constant of 7.90 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-mFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human NKG2A&CD94, mFc Tag captured on CM5 Chip via Anti-mouse Antibody can bind Human HLA-E Complex Tetramer, His Tag with an affinity constant of 7.90 nM as determined in SPR assay (Biacore T200).
Immobilized Human NKG2A&CD94, mFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA.
Serial dilutions of Anti-NKG2A Antibody were added into Biotinylated Human HLA-E*01:03 Complex Tetramer, His Tag : Human NKG2A&CD94, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 80.0ng/ml.
The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.
Human NKG2A&CD94 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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