Name: Human NKG2A&CD94 Protein (NKC-HM394)
Brand: KactusBio
SKU: NKC-HM394-100UG
Price: 330.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-mFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human NKG2A&CD94, mFc Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). The affinity constant of 7.90 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)

Catalog #NKC-HM394

Size

100UG

500UG

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Product Description	Recombinant Human NKG2A&CD94 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94).
Product Background	The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.
Product Category	Recombinant Protein / Antibody discovery

Protein	NKG2A&CD94
Synonyms	CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94
Accession	P26715-1(NKG2A)&Q13241-1(CD94)
Species	Human
Biotinylated	no
Amino Acid Range	Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)
Molecular Weight	The protein has a predicted MW of 56.9 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human NKG2A&CD94, mFc Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). The affinity constant of 7.90 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-mFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human NKG2A&CD94, mFc Tag captured on CM5 Chip via Anti-mouse Antibody can bind Human HLA-E Complex Tetramer, His Tag with an affinity constant of 7.90 nM as determined in SPR assay (Biacore T200).
	Immobilized Human NKG2A&CD94, mFc Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test).
	Human NKG2A&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Serial dilutions of Anti-NKG2A Antibody were added into Biotinylated Human HLA-E*01:03 Complex Tetramer, His Tag : Human NKG2A&CD94, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.15ug/ml.
	The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.