• Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human NKG2A&CD94, mFc Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). The affinity constant of 7.90 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Ala113-Leu233(NKG2A) & Ser34-Ile179(CD94)
Catalog #NKC-HM394
Price
Regular price $36300
Regular price Sale price $36300
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Size
Avantor VWR Fisher Scientific

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Human NKG2A&CD94 Protein (NKC-HM394) Summary

Product Description

 Recombinant Human NKG2A&CD94 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Ala113-Leu233 (NKG2A) and Ser34-Ile179 (CD94).

Product Background

 The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.

Human NKG2A&CD94 Protein (NKC-HM394) Specifications/Details

Protein
 NKG2A&CD94
Synonyms
 CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala113-Leu233(NKG2A) & Ser34-Ile179(CD94)
Molecular Weight
 The protein has a predicted MW of 56.9 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human NKG2A&CD94, mFc Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). The affinity constant of 7.90 nM as determined in SPR assay (Biacore T200).
Product Tag
 C-mFc
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human NKG2A&CD94, mFc Tag captured on CM5 Chip via Anti-mouse Antibody can bind Human HLA-E Complex Tetramer, His Tag with an affinity constant of 7.90 nM as determined in SPR assay (Biacore T200). Human NKG2A&CD94, mFc Tag captured on CM5 Chip via Anti-mouse Antibody can bind Human HLA-E Complex Tetramer, His Tag with an affinity constant of 7.90 nM as determined in SPR assay (Biacore T200).
Immobilized Human NKG2A&CD94, mFc Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). Immobilized Human NKG2A&CD94, mFc Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test).
Human NKG2A&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human NKG2A&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Serial dilutions of Anti-NKG2A Antibody were added into Biotinylated Human HLA-E*01:03 Complex Tetramer, His Tag : Human NKG2A&CD94, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.15ug/ml. Serial dilutions of Anti-NKG2A Antibody were added into Biotinylated Human HLA-E*01:03 Complex Tetramer, His Tag : Human NKG2A&CD94, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.15ug/ml.
The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC. The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
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