Product Description |
Recombinant Human NKG2A&CD94 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94). |
Product Background |
The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies. |
Product Category |
Recombinant Protein |
Target |
NKG2A&CD94 |
Synonyms |
CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94) |
Molecular Weight |
The protein has a predicted MW of 56.9 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human NKG2A&CD94, mFc Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). The affinity constant of 7.90 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-mFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human NKG2A&CD94, mFc Tag captured on CM5 Chip via Anti-mouse Antibody can bind Human HLA-E Complex Tetramer, His Tag with an affinity constant of 7.90 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human NKG2A&CD94, mFc Tag at 1 ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). |
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Human NKG2A&CD94 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Serial dilutions of Anti-NKG2A Antibody were added into Biotinylated Human HLA-E*01:03 Complex Tetramer, His Tag : Human NKG2A&CD94, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.15ug/ml. |
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The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC. |
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