Human NKG2A&CD94 Protein (NKC-HM394)

  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human NKG2A&CD94, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test). The affinity constant of 7.90 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)

Catalog: NKC-HM394

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Product Description Recombinant Human NKG2A&CD94 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94).

Product Background

The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.

Product Category

Recombinant Protein

Target

NKG2A&CD94
Synonyms CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94

Gene/Protein ID

P26715-1(NKG2A)&&Q13241-1(CD94)

Species

Human

Exact Sequence

Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)
Molecular Weight The protein has a predicted MW of 56.9 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human NKG2A&CD94, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test). The affinity constant of 7.90 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-mFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human NKG2A&CD94, mFc Tag captured on CM5 Chip via Anti-mouse Antibody can bind Human HLA-E Complex Tetramer, His Tag with an affinity constant of 7.90 nM as determined in SPR assay (Biacore T200).
Immobilized Human NKG2A&CD94, mFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 12.4ng/ml determined by ELISA (QC Test).
Serial dilutions of Anti-NKG2A Antibody were added into Biotinylated Human HLA-E*01:03 Complex Tetramer, His Tag : Human NKG2A&CD94, mFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 0.15ug/ml.
The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.
Human NKG2A&CD94 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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