Human NKG2A&CD94 Protein (NKC-HM495)

  • Express System: Expi293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human NKG2A&CD94, His Tag at 0.5_g/ml (100_l/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)

Catalog: NKC-HM495

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Product Description Recombinant Human NKG2A&CD94 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94).
Product Category Recombinant Protein
Target NKG2A&CD94
Synonyms CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94
Gene/Protein ID P26715-1(NKG2A)&Q13241-1(CD94)
Species Human
Exact Sequence Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)
Molecular Weight The protein has a predicted MW of 17.9 kDa (CD94)&18.3 kDa (NKG2A). Due to glycosylation, the protein migrates to 33-48 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human NKG2A&CD94, His Tag at 0.5_g/ml (100_l/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA. See testing image for detail.
Product Tag N-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human NKG2A&CD94, His Tag at 0.5_g/ml (100_l/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 10.1ng/ml determined by ELISA.
Serial dilutions of Anti-NKG2A Antibody, hFc Tag were added into Human NKG2A&CD94, His Tag : Biotinylated Human HLA-E*01:03 Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 31.8ng/ml.
The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.
Human NKG2A&CD94 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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