Human NKG2A&CD94 Protein (NKC-HM495)

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human NKG2A&CD94, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)

Catalog: NKC-HM495

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Product Description	Recombinant Human NKG2A&CD94 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Arg100-Leu233(NKG2A)&Ser34-Ile179(CD94).
Product Background	The ligand-receptor assignment between HLA-G and NKG2A/CD94 is dependent of the amino acid composition in the HLA-G heavy chain. Understanding the biophysical basis of receptor-mediated events that lead to NK cell inhibition would help to remove non-tumor reactive cells and support personalized mild autologous NK cell therapies.
Product Category	Recombinant Protein

Target	NKG2A&CD94
Synonyms	CD159a; NKG2A; NKG2-A; CD94; NKG2A&CD94
Gene/Protein ID	P26715-1(NKG2A)&Q13241-1(CD94)
Species	Human
Exact Sequence	Arg100-Leu233(NKG2A) & Ser34-Ile179(CD94)
Molecular Weight	The protein has a predicted MW of 17.9 kDa (CD94)&18.3 kDa (NKG2A). Due to glycosylation, the protein migrates to 33-48 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human NKG2A&CD94, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human NKG2A&CD94, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2A Antibody, hFc Tag with the EC50 of 7.9ng/ml determined by ELISA.
	Serial dilutions of Anti-NKG2A Antibody, hFc Tag were added into Human NKG2A&CD94, His Tag : Biotinylated Human HLA-E*01:03 Complex Tetramer, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 31.8ng/ml.
	The purity of Human NKG2A&CD94 is greater than 95% as determined by SEC-HPLC.
	Human NKG2A&CD94 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.