Human NKG2C&CD94 Protein (NKC-HM696)

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 1.65 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Glu98-Leu231(NKG2C) & Ser34-Ile179(CD94)

Catalog: NKC-HM696

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Product Description	Recombinant Human NKG2C&CD94 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Glu98-Leu231(NKG2C)&Ser34-Ile179(CD94).
Product Background	NKG2C&CD94 is a C-type lectin heterodimer on NK cells and CD8+ cytotoxic T-cells, it can recognize peptides derived from the intracellular proteins in the context of HLA-E. NKG2C&CD94 itself has no signal transduction function but is an activating receptor on the surface of NK cells that involved in driving the NK-cell expansion.
Product Category	Recombinant Protein

Target	NKG2C&CD94
Synonyms	NKG2C&CD94
Gene/Protein ID	P26717(NKG2C)&Q13241-1(CD94)
Species	Human
Exact Sequence	Glu98-Leu231(NKG2C) & Ser34-Ile179(CD94)
Molecular Weight	The protein has a predicted MW of 15.3kDa (NKG2C)&17.9kDa (CD94). Due to glycosylation, the protein migrates to 30-50 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 1.65 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human NKG2C&CD94, His Tag immobilized on CM5 Chip can bind Human HLA-E*01:03 Complex Tetramer, His Tag with an affinity constant of 1.65 uM as determined in SPR assay (Biacore T200).
	The purity of Human NKG2C&CD94 is greater than 95% as determined by SEC-HPLC.
	Human NKG2C&CD94 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.