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Non-biotinylated Human NKG2C&CD94 Protein (NKC-HM696) Summary
Product Description |
Recombinant Human NKG2C&CD94 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus of NKG2C and Flag tag at the N-Terminus of CD94.It contains Glu98-Leu231(NKG2C) and Ser34-Ile179(CD94). |
Product Background |
NKG2C&CD94 is a C-type lectin heterodimer on NK cells and CD8+ cytotoxic T-cells, it can recognize peptides derived from the intracellular proteins in the context of HLA-E. NKG2C&CD94 itself has no signal transduction function but is an activating receptor on the surface of NK cells that involved in driving the NK-cell expansion. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Non-biotinylated Human NKG2C&CD94 Protein (NKC-HM696) Specifications/Details
Protein |
NKG2C&CD94 |
Synonyms |
NKG2C&CD94; NKG2C; CD94 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu98-Leu231(NKG2C) & Ser34-Ile179(CD94) |
Molecular Weight |
The protein has a predicted MW of 15.3kDa (NKG2C)&17.9kDa (CD94). Due to glycosylation, the protein migrates to 30-50 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 1.65 uM as determined in SPR assay (Biacore T200). |
Product Tag |
N-His-Avi, N-Flag |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human NKG2C&CD94, His Tag immobilized on CM5 Chip can bind Human HLA-E*01:03 Complex Tetramer, His Tag with an affinity constant of 1.65 uM as determined in SPR assay (Biacore T200). |
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Human NKG2C&CD94 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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The purity of Human NKG2C&CD94 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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