Name: Non-biotinylated Human NKG2C&CD94 Protein (NKC-HM696)
Brand: KactusBio
SKU: NKC-HM696-100UG
Price: 346.5 USD
Availability: InStock

Non-biotinylated Human NKG2C&CD94 Protein (NKC-HM696)

Human NKG2C&CD94, His Tag immobilized on CM5 Chip can bind Human HLA-E*01:03 Complex Tetramer, His Tag with an affinity constant of 1.65 uM as determined in SPR assay (Biacore T200).

Human NKG2C&CD94 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of Human NKG2C&CD94 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi, N-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 1.65 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Glu98-Leu231(NKG2C) & Ser34-Ile179(CD94)

Catalog #NKC-HM696

Size

100UG

500UG

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Product Description	Recombinant Human NKG2C&CD94 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus of NKG2C and Flag tag at the N-Terminus of CD94.It contains Glu98-Leu231(NKG2C) and Ser34-Ile179(CD94).
Product Background	NKG2C&CD94 is a C-type lectin heterodimer on NK cells and CD8+ cytotoxic T-cells, it can recognize peptides derived from the intracellular proteins in the context of HLA-E. NKG2C&CD94 itself has no signal transduction function but is an activating receptor on the surface of NK cells that involved in driving the NK-cell expansion.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	NKG2C&CD94
Synonyms	NKG2C&CD94; NKG2C; CD94
Accession	P26717(NKG2C)&Q13241-1(CD94)
Species	Human
Biotinylated	no
Amino Acid Range	Glu98-Leu231(NKG2C) & Ser34-Ile179(CD94)
Molecular Weight	The protein has a predicted MW of 15.3kDa (NKG2C)&17.9kDa (CD94). Due to glycosylation, the protein migrates to 30-50 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 1.65 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Avi, N-Flag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human NKG2C&CD94, His Tag immobilized on CM5 Chip can bind Human HLA-E*01:03 Complex Tetramer, His Tag with an affinity constant of 1.65 uM as determined in SPR assay (Biacore T200).
	Human NKG2C&CD94 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of Human NKG2C&CD94 is greater than 95% as determined by SEC-HPLC.