Product Description |
Recombinant Human NKG2D/CD314 Protein is expressed from HEK293 with hFc tag and Flag tag at the N-Terminus.It contains Phe78-Val216. |
Product Background |
NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells. |
Product Category |
Recombinant Protein |
Target |
NKG2D/CD314 |
Synonyms |
CD314; D12S2489E; KLR; NKG2-D; NKG2D |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Phe78-Val216 |
Molecular Weight |
The protein has a predicted MW of 43.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human MICA, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-hFc-Flag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human MICA, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test). |
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Serial dilutions of Anti-NKG2D Antibody, hFc Tag were added into Biotinylated Human ULBP-2, His Tag : Human NKG2D, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 45.2ng/ml. |
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Human NKG2D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human NKG2D is greater than 95% as determined by SEC-HPLC. |
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