Human NKG2D/CD314 Protein (NKG-HM22D)

Express System: HEK293
Product Tag: N-hFc-Flag
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human ULBP-2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 2.8ug/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Phe78-Val216

Catalog: NKG-HM22D

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Product Description	Recombinant Human NKG2D/CD314 Protein is expressed from HEK293 with hFc tag and Flag tag at the N-Terminus.It contains Phe78-Val216.
Product Background	NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
Product Category	Recombinant Protein

Target	NKG2D/CD314
Synonyms	CD314; D12S2489E; KLR; NKG2-D; NKG2D
Gene/Protein ID	P26718
Species	Human
Exact Sequence	Phe78-Val216
Molecular Weight	The protein has a predicted MW of 43.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human ULBP-2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 2.8ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-hFc-Flag
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human ULBP-2, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 2.8ug/ml determined by ELISA (QC Test).
	Immobilized Human MICA, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.88ug/ml determined by ELISA.
	Serial dilutions of Anti-NKG2D Antibody, hFc Tag were added into Biotinylated Human ULBP-2, His Tag : Human NKG2D, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 45.2ng/ml.
	The purity of Human NKG2D is greater than 95% as determined by SEC-HPLC.
	Human NKG2D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.