Human NKG2D/CD314 Protein (NKG-HM22D)

  • Express System: HEK293
  • Product Tag: N-hFc-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human MICA, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Phe78-Val216

Catalog: NKG-HM22D

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Product Description Recombinant Human NKG2D/CD314 Protein is expressed from HEK293 with hFc tag and Flag tag at the N-Terminus.It contains Phe78-Val216.

Product Background

NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.

Product Category

Recombinant Protein

Target

NKG2D/CD314
Synonyms CD314; D12S2489E; KLR; NKG2-D; NKG2D
Accession

Species

Human

Exact Sequence

Phe78-Val216
Molecular Weight The protein has a predicted MW of 43.4 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human MICA, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

N-hFc-Flag

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human MICA, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.81ug/ml determined by ELISA (QC Test).
Serial dilutions of Anti-NKG2D Antibody, hFc Tag were added into Biotinylated Human ULBP-2, His Tag : Human NKG2D, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 45.2ng/ml.
The purity of Human NKG2D is greater than 95% as determined by SEC-HPLC.
Human NKG2D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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