Human NKG2D/CD314 Protein (NKG-HM42D)
Catalog: NKG-HM42D
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Product Description | Recombinant Human NKG2D/CD314 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Phe78-Val216. |
Product Category | Recombinant Protein |
Target | NKG2D/CD314 |
Synonyms | CD314; D12S2489E; KLR; NKG2-D; NKG2D |
Gene/Protein ID | P26718 |
Species | Human |
Exact Sequence | Phe78-Val216 |
Molecular Weight | The protein has a predicted MW of 19.0 kDa. Due to glycosylation, the protein migrates to 36-38 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human NKG2D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. The affinity constant of 95.15 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag | N-His-Avi |
Express System | HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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