Human NKG2D/CD314 Protein (NKG-HM42D)
|Recombinant Human NKG2D/CD314 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Phe78-Val216.
|NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
|CD314; D12S2489E; KLR; NKG2-D; NKG2D
|The protein has a predicted MW of 19.0 kDa. Due to glycosylation, the protein migrates to 36-38 kDa based on Tris-Bis PAGE result.
|Immobilized Human NKG2D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA. The affinity constant of 95.15 nM as determined in SPR assay (Biacore T200). See testing image for detail.
|> 95% as determined by Tris-Bis PAGE
|Less than 1EU per ug by the LAL method.
|Shipped at ambient temperature.
|Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
|Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
|Stability And Storage
|Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.