Non-biotinylated Human NKG2D/CD314 Protein (NKG-HM42D)

SKU: NKG-HM42D-100UG

Non-biotinylated Human NKG2D/CD314 Protein (NKG-HM42D)

SKU: NKG-HM42D-100UG
Non-biotinylated Human NKG2D/CD314 Protein (NKG-HM42D)
Non-biotinylated Human NKG2D/CD314 Protein (NKG-HM42D)
Non-biotinylated Human NKG2D/CD314 Protein (NKG-HM42D)
Non-biotinylated Human NKG2D/CD314 Protein (NKG-HM42D)
  • Express System: HEK293
  • Product Tag: N-Avi-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human NKG2D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA (QC Test). The affinity constant of 95.15 nM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Phe78-Val216
Catalog #NKG-HM42D
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Size
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Non-biotinylated Human NKG2D/CD314 Protein (NKG-HM42D) Summary

Product Description

 Recombinant Human NKG2D/CD314 Protein is expressed from HEK293 with Avi tag and His tag at the N-Terminus.It contains Phe78-Val216.

Product Background

 NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.

Product Category

 Recombinant Protein / Antibody discovery / CAR-T targets

Non-biotinylated Human NKG2D/CD314 Protein (NKG-HM42D) Specifications/Details

Protein
 NKG2D/CD314
Synonyms
 CD314; D12S2489E; KLR; NKG2-D; NKG2D
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Phe78-Val216
Molecular Weight
 The protein has a predicted MW of 19.0 kDa. Due to glycosylation, the protein migrates to 36-38 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human NKG2D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA (QC Test). The affinity constant of 95.15 nM as determined in SPR assay (Biacore T200).
Product Tag
 N-Avi-His

Fluorophore

 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human MICB, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 95.15 nM as determined in SPR assay (Biacore T200). Human MICB, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 95.15 nM as determined in SPR assay (Biacore T200).
Immobilized Human NKG2D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA (QC Test). Immobilized Human NKG2D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA (QC Test).
Immobilized Human NKG2D, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA. Immobilized Human NKG2D, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA.
Immobilized Human NKG2D, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ULBP-6, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. Immobilized Human NKG2D, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ULBP-6, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA.
Human NKG2D on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Human NKG2D on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Loaded Human ULBP-2, hFc Tag (Cat. ULB-HM202) on ProA-Biosensor can bind Human NKG2D, His Tag with an affinity constant of 1.16 µM as determined in BLI assay . Loaded Human ULBP-2, hFc Tag (Cat. ULB-HM202) on ProA-Biosensor can bind Human NKG2D, His Tag with an affinity constant of 1.16 µM as determined in BLI assay .
The purity of Human NKG2D is greater than 90% as determined by SEC-HPLC. The purity of Human NKG2D is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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