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Human NKp30/NCR3/CD337 Protein (NKP-HM230) Summary
Product Description |
Recombinant Human NKp30/NCR3/CD337 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Leu19-Thr138. |
Product Background |
NKp30, along with NKp44 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors". These receptors play a major role in triggering NK-mediated killing of most tumor cells lines.NKp30 stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells. Engagement of NCR3 by BAG6 also promotes myeloid dendritic cells (DC) maturation, both through killing DCs that did not acquire a mature phenotype, and inducing the release by NK cells of TNFA and IFNG which promote DC maturation. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery/Others |
Human NKp30/NCR3/CD337 Protein (NKP-HM230) Specifications/Details
Protein |
NKp30/NCR3/CD337 |
Synonyms |
CD337; LY117; NCR3; NKp30; 1C7; MALS |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu19-Thr138 |
Molecular Weight |
The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human NKp30, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-NKp30 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA (QC Test). The affinity constant of 48.67 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-hFc (IgG1) |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Human B7-H6, His Tag (Cat. BH7-HM176) with an affinity constant of 48.67 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human NKp30, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-NKp30 Antibody, hFc Tag with the EC50 of 2.5ng/ml determined by ELISA (QC Test). |
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Human NKp30 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Human NKp30, hFc Tag on Anti-His-Biosensor can bind Human B7-H6, His Tag (Cat. BH7-HM176) with an affinity constant of 0.56 µM as determined in BLI assay . |
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The purity of Human NKp30 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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