Human NKp30/NCR3/CD337 Protein (NKP-HM230)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human NKp30, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-NKp30 Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test). The affinity constant of 23.10 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Leu19-Thr138

Catalog: NKP-HM230

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Product Description Recombinant Human NKp30/NCR3/CD337 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu19-Thr138.

Product Background

NKp30, along with NKp44 and NKp46, constitute a group of receptors termed "Natural Cytotoxicity Receptors". These receptors play a major role in triggering NK-mediated killing of most tumor cells lines.NKp30 stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells. Engagement of NCR3 by BAG6 also promotes myeloid dendritic cells (DC) maturation, both through killing DCs that did not acquire a mature phenotype, and inducing the release by NK cells of TNFA and IFNG which promote DC maturation.

Product Category

Recombinant Protein

Target

NKp30/NCR3/CD337
Synonyms CD337; LY117; NCR3; NKp30; 1C7; MALS

Gene/Protein ID

AAH52582

Species

Human

Exact Sequence

Leu19-Thr138
Molecular Weight The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human NKp30, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-NKp30 Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test). The affinity constant of 23.10 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Human B7-H6, His Tag with an affinity constant of 23.10 nM as determined in SPR assay (Biacore T200).
Immobilized Human NKp30, hFc Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-NKp30 Antibody, hFc Tag with the EC50 of 18.0ng/ml determined by ELISA (QC Test).
The purity of Human NKp30 is greater than 95% as determined by SEC-HPLC.
Human NKp30 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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