Product Description |
Recombinant Human NKp46/NCR1/CD335 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln22-Asn254. |
Product Background |
NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR). These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
NKp46/NCR1/CD335 |
Synonyms |
CD335; Ly94; NCR1; NKp46; MAR-1; NKP46FLJ99094 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln22-Asn254 |
Molecular Weight |
The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 38-48 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human NKp46, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-Nkp46 Antibody, hFc Tag with the EC50 of 15.0 ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His |
Expression System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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