Human NKp46/NCR1/CD335 Protein (NKP-HM246)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human NKp46, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Nkp46 Antibody, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln22-Asn255

Catalog: NKP-HM246

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Product Description	Recombinant Human NKp46/NCR1/CD335 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-Asn255.
Product Background	NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR).These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells.
Product Category	Recombinant Protein

Target	NKp46/NCR1/CD335
Synonyms	CD335; Ly94; NCR1; NKp46; MAR-1; NKP46FLJ99094
Gene/Protein ID	O76036-1
Species	Human
Exact Sequence	Gln22-Asn255
Molecular Weight	The protein has a predicted MW of 53.2 kDa. Due to glycosylation, the protein migrates to 68-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human NKp46, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Nkp46 Antibody, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human NKp46, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Nkp46 Antibody, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA.
	The purity of Human NKp46 is greater than 95% as determined by SEC-HPLC.
	Human NKp46 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.