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Human NKp46/NCR1/CD335 Protein, Ultra Low Endotoxin (NKP-HM346-UL) Summary
Product Description |
Recombinant Human NKp46/NCR1/CD335 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Gln22-Asn255. |
Product Background |
NKp46, along with NKp30 and NKp44, are activating receptors that have been collectively termed the natural cytotoxicity receptors (NCR).These receptors lack significant sequence homology to one another. They are expressed almost exclusively by NK cells and play a major role in triggering some of the key lytic activities of NK cells. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human NKp46/NCR1/CD335 Protein, Ultra Low Endotoxin (NKP-HM346-UL) Specifications/Details
Protein |
NKp46/NCR1/CD335 |
Synonyms |
CD335; Ly94; NCR1; NKp46; MAR-1; NKP46FLJ99094 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln22-Asn255 |
Molecular Weight |
The protein has a predicted MW of 52.9 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human NKp46, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Nkp46 Antibody, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA (QC Test). |
Product Tag |
C-mFc |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 0.01 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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|
Immobilized Human NKp46, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Nkp46 Antibody, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA (QC Test). |
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Human NKp46 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human NKp46 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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