Human Notch 1 Protein (NOT-HM401)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.48 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ala19-Gln526

Catalog: NOT-HM401

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Product Description Recombinant Human Notch 1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Ala19-Gln526.
Product Category Recombinant Protein
Target Notch 1
Synonyms Notch-1; TAN1; NOTCH1; 9930111A19Rik; lin-12; Mis6; N1
Gene/Protein ID P46531
Species Human
Exact Sequence Ala19-Gln526
Molecular Weight The protein has a predicted MW of 56.5 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Activity The affinity constant of 0.48 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human DLL4, hFc Tag captured on CM5 Chip via Protein A can bind Human Notch 1, His Tag with an affinity constant of 0.48 μM as determined in SPR assay (Biacore T200).
The purity of Human Notch 1 is greater than 95% as determined by SEC-HPLC.
Human Notch 1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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