Human Notch 3 Protein (NOT-HM103)
Human Notch 3 Protein (NOT-HM103)
Human Notch 3 Protein (NOT-HM103)
Human Notch 3 Protein (NOT-HM103)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Notch 3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ala1378-Ser1640
Catalog #NOT-HM103
Regular price $346.50
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Product Description

Recombinant Human Notch 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala1378-Ser1640.

Product Background

Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes. The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Product Category

Recombinant Protein / Antibody discovery

Protein

Notch 3

Synonyms

CADASIL; Notch homolog 3; Notch-3; NOTCH3

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Ala1378-Ser1640

Molecular Weight

The protein has a predicted MW of 30.18 kDa. Due to furin-like protease cleavage and glycosylation, the protein migrates to 27-30 kDa and 10-12 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human Notch 3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human Notch 3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test). Immobilized Human Notch 3, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA (QC Test).
Human Notch 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human Notch 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Notch 3 is greater than 95% as determined by SEC-HPLC. The purity of Human Notch 3 is greater than 95% as determined by SEC-HPLC.

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