Human Notch 3 Protein (NOT-HM103)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Notch 3, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala1378-Ser1640

Catalog: NOT-HM103

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Product Description Recombinant Human Notch 3 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Ala1378-Ser1640.
Product Category Recombinant Protein
Target Notch 3
Gene/Protein ID Q9UM47
Species Human
Exact Sequence Ala1378-Ser1640
Molecular Weight The protein has a predicted MW of 30.18 kDa. Due to furin-like protease cleavage and glycosylation, the protein migrates to 27-30 kDa and 10-12 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Notch 3, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Notch 3, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA.
The purity of Human Notch 3 is greater than 95% as determined by SEC-HPLC.
Human Notch 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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