Human Notch 3 Protein (NOT-HM103)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Notch 3, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala1378-Ser1640

Catalog: NOT-HM103

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Product Description	Recombinant Human Notch 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala1378-Ser1640.
Product Background	Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes. The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Product Category	Recombinant Protein

Target	Notch 3
Gene/Protein ID	Q9UM47
Species	Human
Exact Sequence	Ala1378-Ser1640
Molecular Weight	The protein has a predicted MW of 30.18 kDa. Due to furin-like protease cleavage and glycosylation, the protein migrates to 27-30 kDa and 10-12 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Notch 3, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human Notch 3, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA.
	The purity of Human Notch 3 is greater than 95% as determined by SEC-HPLC.
	Human Notch 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.