Product Description |
Recombinant Human Notch 3 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala40-Glu467. |
Product Background |
Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes. The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
Notch 3 |
Synonyms |
CADASIL; Notch homolog 3; Notch-3; NOTCH3 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala40-Glu467 |
Molecular Weight |
The protein has a predicted MW of 47.7 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human Notch 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human Notch 3 is greater than 95% as determined by SEC-HPLC. |
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