Human Notch 3 Protein (NOT-HM403)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Exact Sequence: Ala40-Glu467

Catalog: NOT-HM403

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Product Description Recombinant Human Notch 3 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Ala40-Glu467.
Product Category Recombinant Protein
Target Notch 3
Synonyms CADASIL; Notch homolog 3; Notch-3; NOTCH3
Gene/Protein ID Q9UM47
Species Human
Exact Sequence Ala40-Glu467
Molecular Weight The protein has a predicted MW of 47.7 kDa. Due to glycosylation, the protein migrates to 53-60 kDa based on Tris-Bis PAGE result.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Human Notch 3 is greater than 95% as determined by SEC-HPLC.
Human Notch 3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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