Human Notch 3 Protein (NOT-HM203) Summary
Product Description |
Recombinant Human Notch 3 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala40-Glu467. |
Product Background |
Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes. The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human Notch 3 Protein (NOT-HM203) Specifications/Details
Protein |
Notch 3 |
Synonyms |
CADASIL; Notch homolog 3; Notch3; Notch-3 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala40-Glu467 |
Molecular Weight |
The protein has a predicted MW of 71.53 kDa. Due to glycosylation, the protein migrates to 75-83 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Human Notch 3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human Notch 3 is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
More from Antibody Drug Discovery Proteins
Recently viewed
