Name: Human NPR1/NPRA Protein (NPR-HM301)
Brand: KactusBio
SKU: NPR-HM301-100UG
Price: 345.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-mFc
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human NPR1, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NPR1 Antibody, hFc Tag with the EC50 of 21.6ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gly33-Glu473

Catalog #NPR-HM301

Size

100UG

500UG

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Product Description	Recombinant Human NPR1/NPRA Protein is expressed from HEK293 with mFc (IgG1) tag at the C-terminus.It contains Gly33-Glu473.
Product Background	NPR1 (natriuretic peptide receptor 1), a receptor of ANP (atrial natriuretic peptide) whitch acting through NPR1, provokes hypotension. NPR1 was abundantly expressed in endothelial cells and smooth muscle cells of small arteries and arterioles. NPR1 plays a crucial role in ANP-mediated blood pressure regulation, presumably by a mechanism that is RGS2-dependent in the acute phase and RGS2-independent in the chronic phase.
Product Category	Recombinant Protein / Antibody discovery

Protein	NPR1/NPRA
Synonyms	ANP-A; ANPR-A; NPR-A; GC-A; ANPRA
Accession	NP_000897.3
Species	Human
Biotinylated	no
Amino Acid Range	Gly33-Glu473
Molecular Weight	The protein has a predicted MW of 74.56 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human NPR1, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NPR1 Antibody, hFc Tag with the EC50 of 21.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-mFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human NPR1, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NPR1 Antibody, hFc Tag with the EC50 of 21.6ng/ml determined by ELISA.
	Human NPR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human NPR1 is greater than 90% as determined by SEC-HPLC.