Name: Human NPR1/NPRA Protein (NPR-HM301)
Brand: KactusBio
SKU: NPR-HM301-100UG
Price: 379.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-mFc
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human NPR1, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NPR1 Antibody, hFc Tag with the EC50 of 43.2ng/ml determined by ELISA (QC Test).
Amino Acid Range: Gly33-Glu473
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Catalog #NPR-HM301

Size

100UG

500UG

Order by 4pm ET for delivery on:

Human NPR1/NPRA Protein (NPR-HM301) Summary

Product Description	Recombinant Human NPR1/NPRA Protein is expressed from HEK293 with mFc (IgG1) tag at the C-terminus.It contains Gly33-Glu473.
Product Background	NPR1 (natriuretic peptide receptor 1), a receptor of ANP (atrial natriuretic peptide) whitch acting through NPR1, provokes hypotension. NPR1 was abundantly expressed in endothelial cells and smooth muscle cells of small arteries and arterioles. NPR1 plays a crucial role in ANP-mediated blood pressure regulation, presumably by a mechanism that is RGS2-dependent in the acute phase and RGS2-independent in the chronic phase.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human NPR1/NPRA Protein (NPR-HM301) Specifications/Details

Protein	NPR1/NPRA
Synonyms	ANP-A; ANPR-A; NPR-A; GC-A; ANPRA
Accession	NP_000897.3
Species	Human
Biotinylated	no
Amino Acid Range	Gly33-Glu473
Molecular Weight	The protein has a predicted MW of 74.56 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human NPR1, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NPR1 Antibody, hFc Tag with the EC50 of 43.2ng/ml determined by ELISA (QC Test).
Product Tag	C-mFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human NPR1, mFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NPR1 Antibody, hFc Tag with the EC50 of 43.2ng/ml determined by ELISA (QC Test).
	Human NPR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human NPR1, mFc Tag on Anti-Mouse IgG Fc-Biosensor can bind Anti-NPR1 Antibody, hFc-Avi Tag with an affinity constant of 1.20 nM as determined in BLI assay (Gator® Prime).
	The purity of Human NPR1 is greater than 90% as determined by SEC-HPLC.