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Human NRAS-Protein (NRS-HE101) Summary
Product Description |
Recombinant Human NRAS-Protein is expressed from E.coli with His tag at the N-Terminus.It contains Met1-Cys186. |
Product Background |
NRas is a key mediator of the mitogenic pathway in normal cells and in cancer cells. Its dynamics and nanoscale organization at the plasma membrane (PM) facilitate its signaling. NRas activity is mediated and regulated by its dynamic interactions with functional (Grb2, BRAF, NF1, and GPI-nucleated) clusters at the PM. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human NRAS-Protein (NRS-HE101) Specifications/Details
Protein |
NRAS |
Synonyms |
GTPase NRas; N-Ras; HRAS1; ALPS4; CMNS; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met1-Cys186 |
Molecular Weight |
The protein has a predicted MW of 22.29 kDa same as Bis-Tris PAGE result. |
Product Tag |
N-His |
Fluorophore |
Unconjugated |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Human NRAS on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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