Product Description |
Recombinant Human NRAS-Protein is expressed from E.coli with His tag at the N-Terminus.It contains Met1-Cys186. |
Product Background |
NRas is a key mediator of the mitogenic pathway in normal cells and in cancer cells. Its dynamics and nanoscale organization at the plasma membrane (PM) facilitate its signaling. NRas activity is mediated and regulated by its dynamic interactions with functional (Grb2, BRAF, NF1, and GPI-nucleated) clusters at the PM. |
Product Category |
Recombinant Protein |
Target |
NRAS |
Synonyms |
GTPase NRas; N-Ras; HRAS1; ALPS4; CMNS; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met1-Cys186 |
Molecular Weight |
The protein has a predicted MW of 22.29 kDa same as Bis-Tris PAGE result. |
Product Tag |
N-His |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human NRAS on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human NRAS-is greater than 90% as determined by SEC-HPLC. |
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