• Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human NRG1 Beta 1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Her3, hFc Tag (Cat. HER-HM203) with the EC50 of 35.8ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Ser177-Glu241
Catalog #NRG-HM1B1
Price
Regular price $23980
Regular price Sale price $23980
/
Size
Avantor VWR Fisher Scientific

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Human NRG1 Beta 1 Protein (NRG-HM1B1) Summary

Product Description

 Recombinant Human NRG1 Beta 1 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Ser177-Glu241.

Product Background

 Neuregulin-1 (NRG-1) is a ligand of the epidermal growth factor receptor (erbB), and its interaction involves activation of the glutamatergic N-methyl-Daspartate receptor, which increases the expression of the β2 subunit of the γ- aminobutyric acid receptor and subunits of the nicotinic acetylcholine receptor.

Human NRG1 Beta 1 Protein (NRG-HM1B1) Specifications/Details

Protein
 NRG1 Beta 1
Synonyms
 Pro-NRG1; NRG1; GGF; HGL; HRGA; NDF; SMDF; Beta1; Beta1A
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ser177-Glu241
Molecular Weight
 The protein has a predicted MW of 8.57 kDa. Due to glycosylation, the protein migrates to 10-13 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human NRG1 Beta 1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Her3, hFc Tag (Cat. HER-HM203) with the EC50 of 35.8ng/ml determined by ELISA (QC Test).
Product Tag
 N-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 50mM NaAc, 150mM NaCl (pH 5.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.； -80°C for 3 months after reconstitution.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human NRG1 Beta 1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Her3, hFc Tag (Cat. HER-HM203) with the EC50 of 35.8ng/ml determined by ELISA (QC Test). Immobilized Human NRG1 Beta 1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Her3, hFc Tag (Cat. HER-HM203) with the EC50 of 35.8ng/ml determined by ELISA (QC Test).
Immobilized Human NRG1 Beta 1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Her4, hFc Tag with the EC50 of 33.4ng/ml determined by ELISA. Immobilized Human NRG1 Beta 1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Her4, hFc Tag with the EC50 of 33.4ng/ml determined by ELISA.
Human NRG1 Beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human NRG1 Beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Human NRG1 beta 1, His Tag on Anti-His-Biosensor can bind Human Her3, hFc Tag (Cat. HER-HM203) with an affinity constant of 2.61 nM as determined in BLI assay. Loaded Human NRG1 beta 1, His Tag on Anti-His-Biosensor can bind Human Her3, hFc Tag (Cat. HER-HM203) with an affinity constant of 2.61 nM as determined in BLI assay.
Other Available Proteins:
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