Human NY-ESO-1 (HLA-A*02:01) Protein (MHC-HM405)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-NY-ESO-1 (HLA-A*02:01) Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human NY-ESO-1 (HLA-A*02:01) , His Tag with the EC50 of 27.1ng/ml determined by ELISA. The affinity constant of 13.43 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and SLLMWITQC peptide

Catalog: MHC-HM405

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Product Description Recombinant Human NY-ESO-1 (HLA-A*02:01) Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and SLLMWITQC peptide.
Product Category Recombinant Protein
Target NY-ESO-1 (HLA-A*02:01)
Synonyms MHC; MY-ESO-1; CT6.1; LAGE-2; CTAG1; CTAG1B; ESO1CTAG; LAGE2A; NY-ESO-1
Gene/Protein ID A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLMWITQC
Species Human
Exact Sequence Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and SLLMWITQC peptide
Molecular Weight The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 51-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-NY-ESO-1 (HLA-A*02:01) Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human NY-ESO-1 (HLA-A*02:01) , His Tag with the EC50 of 27.1ng/ml determined by ELISA. The affinity constant of 13.43 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22μm filtered solution in PBS, 100mM L-arginine (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
Anti-NY-ESO-1 (HLA-A*02:01) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human NY-ESO-1 (HLA-A*02:01) , His Tag with an affinity constant of 13.43 nM as determined in SPR assay (Biacore T200).
Immobilized Anti-NY-ESO-1 (HLA-A*02:01) Antibody, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human NY-ESO-1 (HLA-A*02:01) , His Tag with the EC50 of 27.1ng/ml determined by ELISA.
The purity of Human NY-ESO-1 (HLA-A*02:01) was greater than 95% as determined by SEC-HPLC.
Human NY-ESO-1 (HLA-A*02:01) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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