Human NY-ESO-1 (HLA-A*02:01) Complex Tetramer Protein (MHC-HM405T)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human NY-ESO-1 (HLA-A*02:01) Complex Tetramer, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-NY-ESO-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. The affinity constant of 0.15 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and SLLMWITQC peptide

Catalog: MHC-HM405T

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Product Description Recombinant Human NY-ESO-1 (HLA-A*02:01) Complex Tetramer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and SLLMWITQC peptide.
Product Category Recombinant Protein
Target NY-ESO-1 (HLA-A*02:01) Complex Tetramer
Synonyms MHC; MY-ESO-1; CT6.1; LAGE-2; CTAG1; CTAG1B; ESO1CTAG; LAGE2A; NY-ESO-1
Gene/Protein ID A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLMWITQC
Species Human
Exact Sequence Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and SLLMWITQC peptide
Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Activity Immobilized Human NY-ESO-1 (HLA-A*02:01) Complex Tetramer, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-NY-ESO-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. The affinity constant of 0.15 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22μm filtered solution in PBS (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
Anti-hFc IgG capture Anti-NY-ESO-1 (HLA-A*02:01) Antibody, Human NY-ESO-1 (HLA-A*02:01) Complex Tetramer as analyte; The affinity constant of 0.15 nM as determined in SPR assay (Biacore T200).
Immobilized Human NY-ESO-1 (HLA-A*02:01) Complex Tetramer, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-NY-ESO-1 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
The purity of Human NY-ESO-1 (HLA-A*02:01) Complex Tetramer was greater than 95% as determined by SEC-HPLC.
Human NY-ESO-1 (HLA-A*02:01) Complex Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

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