• Express System: E.coli
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody at 5ug/ml (100ul/well) on the plate. Dose response curve for Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with the EC50 of 7.2ng/ml determined by ELISA.
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLMWITQC peptide
Catalog #MHC-HE446
Price
Regular price $37180
Regular price Sale price $37180
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Size
Avantor VWR Fisher Scientific

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Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein (MHC-HE446) Summary

Product Description

 Recombinant Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLMWITQC peptide.

Product Background

 NY-ESO-1 or New York esophageal squamous cell carcinoma 1 is a well-known cancer-testis antigen (CTAs) with re-expression in numerous cancer types. Its ability to elicit spontaneous humoral and cellular immune responses, together with its restricted expression pattern, have rendered it a good candidate target for cancer immunotherapy.

HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Specifications

Protein
 HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC)
Synonyms
 MHC; MY-ESO-1; CT6.1; LAGE-2; CTAG1; CTAG1B; ESO1CTAG; LAGE2A; NY-ESO-1; CTAG1A;MY-ESO1; LAGE2; NY-ESO1
MHC Form
 Monomer
Allele
 HLA-A*02:01
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLMWITQC peptide
Molecular Weight
 The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Activity
 Immobilized Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody at 5ug/ml (100ul/well) on the plate. Dose response curve for Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with the EC50 of 7.2ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Unconjugated
Expression System
 E.coli
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Liquid
Shipping
 Shipped with dry ice.
Formulation
 Supplied as 0.22um filtered solution in 20mM Tris, 200mM NaCl (pH 8.0).
Stability And Storage
 Valid for 12 months from date of receipt when stored at -80°C.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody at 5ug/ml (100ul/well) on the plate. Dose response curve for Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with the EC50 of 7.2ng/ml determined by ELISA. Immobilized Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody at 5ug/ml (100ul/well) on the plate. Dose response curve for Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer, His Tag with the EC50 of 7.2ng/ml determined by ELISA.
Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer is greater than 95% as determined by SEC-HPLC. The purity of Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Monomer is greater than 95% as determined by SEC-HPLC.
Additional Information
Learn More: MHC Collection Learn More: Peptide-ready MHC Protocol: MHC Staining Case Study: Peptide-ready MHC for TCR Staining Poster: Engineering MHCs as Versatile Reagents Publication: Use of our Peptide-Ready MHCs in CAR-T Cell Research Product Brochure
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