Product Description |
Recombinant Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein is expressed from E.coli with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and SLLMWITQC peptide. |
Product Background |
NY-ESO-1 or New York esophageal squamous cell carcinoma 1 is a well-known cancer-testis antigen (CTAs) with re-expression in numerous cancer types. Its ability to elicit spontaneous humoral and cellular immune responses, together with its restricted expression pattern, have rendered it a good candidate target for cancer immunotherapy. |
Product Category |
Recombinant Protein |
Target |
HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer |
MHC Form |
Tetramer |
Allele |
HLA-A*02:01 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLMWITQC peptide |
Molecular Weight |
The protein has a predicted MW of 258 kDa. The protein migrates to 120-180 kDa (HLA-A*02:01&streptavidin tetramer) and 11.9 kDa (B2M) based on Bis-Tris PAGE result under Non reducing (N) condition. |
Activity |
Immobilized Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. |
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Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%. |
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The purity of Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer is greater than 95% as determined by SEC-HPLC. |
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