Name: Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein (MHC-HE446T)
Brand: KactusBio
SKU: MHC-HE446T-100UG
Price: 1125.0 USD
Availability: InStock

Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein (MHC-HE446T)

Immobilized Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA.

Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.

The purity of Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer is greater than 95% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLMWITQC peptide

Catalog #MHC-HE446T

Size

100UG

500UG

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Product Description	Recombinant Human HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein is expressed from E.coli with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305 (HLA-A02:01), Ile21-Met119 (B2M) and SLLMWITQC peptide.
Product Background	NY-ESO-1 or New York esophageal squamous cell carcinoma 1 is a well-known cancer-testis antigen (CTAs) with re-expression in numerous cancer types. Its ability to elicit spontaneous humoral and cellular immune responses, together with its restricted expression pattern, have rendered it a good candidate target for cancer immunotherapy.
Product Category	Recombinant Protein / Antibody discovery / MHC

Protein	HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer
Synonyms	MHC; MY-ESO-1; CT6.1; LAGE-2; CTAG1; CTAG1B; ESO1CTAG; LAGE2A; NY-ESO-1; CTAG1A
MHC Form	Tetramer
Allele	HLA-A*02:01
Accession	A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLMWITQC
Species	Human
Biotinylated	no
Amino Acid Range	Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and SLLMWITQC peptide
Molecular Weight	The protein has a predicted MW of 258 kDa. The protein migrates to 120-180 kDa (HLA-A*02:01&streptavidin tetramer) and 11.9 kDa (B2M) based on Bis-Tris PAGE result under Non reducing (N) condition.
Activity	Immobilized Human HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag with the EC50 of 1.9ng/ml determined by ELISA.
	Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.
	The purity of Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer is greater than 95% as determined by SEC-HPLC.