Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Tetramer Protein (MHC-HM40NT)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Tetramer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and SLLMWITQV peptide

Catalog: MHC-HM40NT

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Product Description Recombinant Human NY-ESO-1(HLA-A*02:01) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and SLLMWITQV peptide.

Product Background

NY-ESO-1 or New York esophageal squamous cell carcinoma 1 is a well-known cancer-testis antigen (CTAs) with re-expression in numerous cancer types. Its ability to elicit spontaneous humoral and cellular immune responses, together with its restricted expression pattern, have rendered it a good candidate target for cancer immunotherapy.

Product Category

Recombinant Protein

Target

HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Tetramer
MHC Form Tetramer
Allele HLA-A*02:01

Gene/Protein ID

A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLMWITQV

Species

Human

Exact Sequence

Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and SLLMWITQV peptide
Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

Activity

Immobilized Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Tetramer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Tetramer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Antibody, hFc Tag with the EC50 of 6.5ng/ml determined by ELISA.
The purity of Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Tetramer is greater than 95% as determined by SEC-HPLC.
Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQV) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

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