Human Oncostatin M/OSM Protein (ONM-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.884 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ala26-Arg221

Catalog: ONM-HM101

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Product Description	Recombinant Human Oncostatin M/OSM Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala26-Arg221.
Product Background	OSM is a pleiotropic cytokine that initiates its biological activities by binding to specific cell surface receptors. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.
Product Category	Recombinant Protein

Target	Oncostatin M/OSM
Gene/Protein ID	P13725
Species	Human
Exact Sequence	Ala26-Arg221
Molecular Weight	The protein has a predicted MW of 23.24 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.884 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human LIF R, hFc Tag captured on CM5 Chip via Protein A can bind Human OSM, His Tag with an affinity constant of 0.884 uM as determined in SPR assay (Biacore T200).
	The purity of Human Oncostatin M is greater than 95% as determined by SEC-HPLC.
	Human Oncostatin M on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.