Human Oncostatin M/OSM Protein (ONM-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 0.884 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala26-Arg221
Catalog #ONM-HM101
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Product Description
 Recombinant Human Oncostatin M/OSM Protein is expressed from HEK293 with His tag at the C-terminus.It contains Ala26-Arg221.
Product Background
 OSM is a pleiotropic cytokine that initiates its biological activities by binding to specific cell surface receptors. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIFR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.
Product Category
 Recombinant Protein
Target
 Oncostatin M/OSM
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala26-Arg221
Molecular Weight
 The protein has a predicted MW of 23.24 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 0.884 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human LIF R, hFc Tag captured on CM5 Chip via Protein A can bind Human OSM, His Tag with an affinity constant of 0.884 uM as determined in SPR assay (Biacore T200).
Human Oncostatin M on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.The ED50-for this effect is 0.3-1 ng/mL (QC Test).
The purity of Human Oncostatin M is greater than 95% as determined by SEC-HPLC.

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