Human OSMR-GPL fusion Protein (OSG-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human OSMR-GPL fusion Protein, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Met1-Ala428(OSMR) & Leu21-Glu225(GPL)

Catalog: OSG-HM101

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Product Description	Recombinant Human OSMR-GPL fusion Protein is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Ala428 (OSMR) and Leu21-Glu225 (GPL).
Product Background	OSMR involvement, together with Gp130-like receptor (GPL), also known as IL-31R, in the formation of a functional IL-31 receptor complex. OSMR-GPL fusion protein was designed to contain the domains of OSMR and GPL required for high affinity IL-31 binding.
Product Category	Recombinant Protein

Target	OSMR-GPL fusion
Gene/Protein ID	Q99650-1(OSMR)&Q8NI17-1(GPL)
Species	Human
Exact Sequence	Met1-Ala428(OSMR) & Leu21-Glu225(GPL)
Molecular Weight	The protein has a predicted MW of 75.84 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human OSMR-GPL fusion Protein, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human OSMR-GPL fusion Protein, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA.
	Human OSMR-GPL fusion Protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.