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Human OSMR beta-GPL fusion Protein (OSG-HM101) Summary
Product Description |
Recombinant Human OSMR beta-GPL fusion Protein is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Ala428 (OSMR beta) and Leu21-Glu225 (GPL). |
Product Background |
OSMR involvement, together with Gp130-like receptor (GPL), also known as IL-31R, in the formation of a functional IL-31 receptor complex. OSMR-GPL fusion protein was designed to contain the domains of OSMR and GPL required for high affinity IL-31 binding. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human OSMR beta-GPL fusion Protein (OSG-HM101) Specifications/Details
Protein |
OSMR beta-GPL fusion |
Synonyms |
OSMR-GPL |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met1-Ala428(OSMR beta) & Leu21-Glu225(GPL) |
Molecular Weight |
The protein has a predicted MW of 75.84 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human OSMR beta-GPL fusion Protein, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Human OSMR beta-GPL fusion Protein, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. |
|
|
Human OSMR beta-GPL fusion Protein on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
Other Available Proteins:
Finding available proteins...
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