Product Description |
Recombinant Human OSMR-GPL fusion Protein is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Ala428 (OSMR) and Leu21-Glu225 (GPL). |
Product Background |
OSMR involvement, together with Gp130-like receptor (GPL), also known as IL-31R, in the formation of a functional IL-31 receptor complex. OSMR-GPL fusion protein was designed to contain the domains of OSMR and GPL required for high affinity IL-31 binding. |
Product Category |
Recombinant Protein |
Target |
OSMR-GPL fusion |
Synonyms |
OSMR-GPL |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met1-Ala428(OSMR) & Leu21-Glu225(GPL) |
Molecular Weight |
The protein has a predicted MW of 75.84 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human OSMR-GPL fusion Protein, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human OSMR-GPL fusion Protein, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. |
|
|
Human OSMR-GPL fusion Protein on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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