ELISA binding curve of human OSMR His Tag protein with anti-OSMR hFc-tagged antibody, demonstrating dose-dependent OD450 signal response for binding affinity analysis.
Human OSMR beta Protein (OSM-HM101)
Human OSMR beta Protein (OSM-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human OSMR beta, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Glu28-Met740
Catalog #OSM-HM101
Regular price $330.00
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Product Description

Recombinant Human OSMR beta Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu28-Met740.

Product Background

OSMR is targeted to the mitochondrial matrix via the presequence translocase-associated motor complex components, mtHSP70 and TIM44. OSMR interacts with NADH ubiquinone oxidoreductase 1/2 (NDUFS1/2) of complex I and promotes mitochondrial respiration. Deletion of OSMR impairs spare respiratory capacity, increases reactive oxygen species, and sensitizes BTSCs to IR-induced cell death.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

OSMR beta

Synonyms

IL-31R subunit beta; IL-31R-beta; IL-31RB; OSMR beta; OSMR; OSMRB; OSMRBMGC150626; PLCA1 ;IL-31R subunit β; IL-31R-β; OSMR β

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Glu28-Met740

Molecular Weight

The protein has a predicted MW of 82.2 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human OSMR beta, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human OSMR beta, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA. Immobilized Human OSMR beta, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA.
Human OSMR beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human OSMR beta on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Anti-OSMR Ab., hFc-Avi Tag on ProA-Biosensor can bind Human OSMR beta, His Tag with an affinity constant of 2.48 nM as determined in BLI assay (Gator® Prime). Loaded Anti-OSMR Ab., hFc-Avi Tag on ProA-Biosensor can bind Human OSMR beta, His Tag with an affinity constant of 2.48 nM as determined in BLI assay (Gator® Prime).
The purity of Human OSMR beta is greater than 95% as determined by SEC-HPLC. The purity of Human OSMR beta is greater than 95% as determined by SEC-HPLC.

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