Human OX40 Ligand/TNFSF4 Protein (OXL-HM240)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human OX40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA (QC Test). The affinity constant of 4.17 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln51-Leu183

Catalog: OXL-HM240

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Product Description	Recombinant Human OX40 Ligand/TNFSF4 Protein is expressed from HEK293 with hFc tag at the N-terminus.It contains Gln51-Leu183.
Product Background	Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation.
Product Category	Recombinant Protein

Target	OX40 Ligand/TNFSF4
Accession	P23510-1
Species	Human
Amino Acid Range	Gln51-Leu183
Molecular Weight	The protein has a predicted MW of 40.95 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Biotinylated Human OX40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA (QC Test). The affinity constant of 4.17 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human OX40, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human OX40 Ligand, hFc Tag with an affinity constant of 4.17 nM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human OX40, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.9ng/ml determined by ELISA (QC Test).
	The purity of Human OX40 Ligand is greater than 95% as determined by SEC-HPLC.
	Human OX40 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.