Human OX40 Ligand/TNFSF4 Trimer Protein (OXL-HM140)
Human OX40 Ligand/TNFSF4 Trimer Protein (OXL-HM140)
Human OX40 Ligand/TNFSF4 Trimer Protein (OXL-HM140)
  • Express System: HEK293
  • Product Tag: N-His-Flag
  • Purity: > 90% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human OX40 Ligand Trimer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 37.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln51-Leu183
Catalog #OXL-HM140
Regular price $300.00
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Product Description
Recombinant Human OX40 Ligand/TNFSF4 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Gln51-Leu183.
Product Background
Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation.
Product Category
Recombinant Protein
Target
OX40 Ligand/TNFSF4 Trimer
Synonyms
OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L;-TXGP1; Glycoprotein Gp34
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Gln51-Leu183
Molecular Weight
The protein has a predicted MW of 49.7 kDa. Due to glycosylation, the protein migrates to 65-140 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human OX40 Ligand Trimer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 37.7ng/ml determined by ELISA. See testing image for detail.
Product Tag
N-His-Flag
Express System
HEK293
Purity
> 90% as determined by Bis-Tris PAGE
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human OX40 Ligand Trimer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 37.7ng/ml determined by ELISA.
Human OX40 Ligand Trimer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 90%.

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