Human OX40 Ligand/TNFSF4 Trimer Protein (OXL-HM140)

  • Express System: Expi293
  • Product Tag: N-His-Flag
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human OX40 Ligand Trimer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA. The affinity constant of 0.98 μM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln51-Leu183

Catalog: OXL-HM140

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Product Description Recombinant Human OX40 Ligand/TNFSF4 Trimer Protein is expressed from Expi293 with His tag and Flag tag at the N-terminal.; It contains Gln51-Leu183.
Product Category Recombinant Protein
Target OX40 Ligand/TNFSF4 Trimer
Synonyms OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L;  TXGP1; Glycoprotein Gp34
Gene/Protein ID P23510-1
Species Human
Exact Sequence Gln51-Leu183
Molecular Weight The protein has a predicted MW of 49.7 kDa. Due to glycosylation, the protein migrates to 65-140 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human OX40 Ligand Trimer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA. The affinity constant of 0.98 μM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag N-His-Flag
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human OX40 Ligand Trimer, His-Flag Tag immobilized on CM5 Chip can bind Human OX40, His Tag with an affinity constant of 0.98 μM as determined in SPR assay (Biacore T200).
Immobilized Human OX40 Ligand Trimer, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA.
The purity of Human OX40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.
Human OX40 Ligand Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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