Human OX40 Ligand/TNFSF4 Trimer Protein (OXL-HM140)

  • Express System: HEK293
  • Product Tag: N-His-Flag
  • Purity: > 90% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human OX40 Ligand Trimer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 37.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln51-Leu183

Catalog: OXL-HM140

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Product Description Recombinant Human OX40 Ligand/TNFSF4 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Gln51-Leu183.

Product Background

 Tumor necrosis factor ligand superfamily member 4 (TNFSF4) is also known as glycoprotein Gp34, OX40 ligand (OX40L),which belongs to the tumor necrosis factor family. It is expressed on such cells as DC2s (a subtype of dendritic cells) enabling amplification of Th2 cell differentiation.

Product Category

 Recombinant Protein

Target

 OX40 Ligand/TNFSF4 Trimer
Synonyms OX40L; OX-40L; OX4OL; CD252; TNFSF4; OX40 Ligand; CD134 ligand; CD134L;-TXGP1; Glycoprotein Gp34

Gene/Protein ID

 P23510-1

Species

 Human

Exact Sequence

 Gln51-Leu183
Molecular Weight The protein has a predicted MW of 49.7 kDa. Due to glycosylation, the protein migrates to 65-140 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Human OX40 Ligand Trimer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 37.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

 N-His-Flag

Express System

 HEK293
Purity > 90% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human OX40 Ligand Trimer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 37.7ng/ml determined by ELISA.
Human OX40 Ligand Trimer on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.

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