Human OX40/TNFRSF4/CD134 Protein (OX4-HM240)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human OX40 Ligand Trimer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 0.15ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Leu29-Ala216

Catalog: OX4-HM240

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Product Description	Recombinant Human OX40 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu29-Ala216.
Product Background	Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.
Product Category	Recombinant Protein

Target	OX40/TNFRSF4/CD134
Synonyms	CD134; OX40; OX40L receptor; TNFRSF4; ACT-135; Ly-70; OX40 homologue;-TXGP1L; IMD16
Gene/Protein ID	P43489
Species	Human
Exact Sequence	Leu29-Ala216
Molecular Weight	The protein has a predicted MW of 46.8 kDa. Due to glycosylation, the protein migrates to 70-78 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human OX40 Ligand Trimer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 0.15ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human OX40 Ligand Trimer, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human OX40, hFc Tag with the EC50 of 0.15ug/ml determined by ELISA.
	Human OX40 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.