Human OX40/TNFRSF4/CD134 Protein (OX4-HM440)
Human OX40/TNFRSF4/CD134 Protein (OX4-HM440)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 4.17 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu29-Ala216
Catalog #OX4-HM440
Regular price $360.00
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Product Description
Recombinant Human OX40/TNFRSF4/CD134 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu29-Ala216.
Product Background
Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.
Product Category
Recombinant Protein
Target
OX40/TNFRSF4/CD134
Synonyms
CD134; OX40; OX40L receptor; TNFRSF4; ACT-135; Ly-70; OX40 homologue;-TXGP1L; IMD16
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Leu29-Ala216
Molecular Weight
The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 4.17 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human OX40, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human OX40 Ligand, hFc Tag with an affinity constant of 4.17 nM as determined in SPR assay (Biacore T200).
Human OX40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human OX40 is greater than 95% as determined by SEC HPLC.

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