Human OX40/TNFRSF4/CD134 Protein (OX4-HM440)
Human OX40/TNFRSF4/CD134 Protein (OX4-HM440)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human OX40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test). The affinity constant of 4.17 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu29-Ala216
Catalog #OX4-HM440
Regular price $360.00
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Product Description

Recombinant Human OX40/TNFRSF4/CD134 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Leu29-Ala216.

Product Background

Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

OX40/TNFRSF4/CD134

Synonyms

CD134; OX40; OX40L receptor; TNFRSF4; ACT-135; Ly-70; OX40 homologue;-TXGP1L; IMD16

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Leu29-Ala216

Molecular Weight

The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human OX40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test). The affinity constant of 4.17 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human OX40, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human OX40 Ligand, hFc Tag with an affinity constant of 4.17 nM as determined in SPR assay (Biacore T200). Human OX40, His-Avi Tag captured on CM5 Chip via Anti-His Antibody can bind Human OX40 Ligand, hFc Tag with an affinity constant of 4.17 nM as determined in SPR assay (Biacore T200).
Immobilized Human OX40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test). Immobilized Human OX40, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human OX40 Ligand, hFc Tag with the EC50 of 3.6ng/ml determined by ELISA (QC Test).
Human OX40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human OX40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human OX40 is greater than 95% as determined by SEC HPLC. The purity of Human OX40 is greater than 95% as determined by SEC HPLC.

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