• Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human IL-1RL1, hFc Tag (Cat. ST2-HM2L1) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-33red, His Tag with the EC50 of 23.5ng/ml and not bind Human IL-33ox, His Tag determined by ELISA.
  • Amino Acid Range: His109-Thr270
Catalog #IL3-HM1OX
Price
Regular price $45000
Regular price Sale price $45000
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Size
Avantor VWR Fisher Scientific

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Human oxidized IL-33/IL-33ox Protein (IL3-HM1OX) Summary

Product Description

 Recombinant Human IL-33ox Protein is expressed from HEK293 with His tag at the N-terminus.It contains His109-Thr270.

Product Background

 Interleukin (IL)-33 released from damaged airway epithelia causes inflammation via its receptor, serum stimulation-2 (ST2). Oxidation of IL-33 to a non-ST2-binding form (IL-33ox) is thought to limit its activity. IL-33ox forms a complex with receptor for advanced glycation end products (RAGE) and epidermal growth factor receptor (EGFR) expressed on airway epithelium. IL-33ox increased the proportion of mucus-producing cells and reduced epithelial defence functions, mimicking pathogenic traits of COPD. Neutralisation of the IL-33ox pathway reversed these deleterious traits in COPD epithelia.

Human oxidized IL-33/IL-33ox Protein (IL3-HM1OX) Specifications/Details

Protein
 IL-33
Synonyms
 C9orf26 Z; DVS27; IL1F11; NF-HEV; NFEHEV; IL33; DV27; C9ORF26; RP11-575C20.2;C9orf26Z;Interleukin-33; Interleukin 33
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 His109-Thr270
Molecular Weight
 The protein has a predicted MW of 19.43 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human IL-1RL1, hFc Tag (Cat. ST2-HM2L1) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-33red, His Tag with the EC50 of 23.5ng/ml and not bind Human IL-33ox, His Tag determined by ELISA.
Product Tag
 N-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
 Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human IL-1RL1, hFc Tag (Cat. ST2-HM2L1) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-33<sup>red</sup>, His Tag with the EC50 of 23.5ng/ml and not bind Human IL-33<sup>ox</sup>, His Tag determined by ELISA. Immobilized Human IL-1RL1, hFc Tag (Cat. ST2-HM2L1) at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-33red, His Tag with the EC50 of 23.5ng/ml and not bind Human IL-33ox, His Tag determined by ELISA.
Immobilized Human IL-33, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Itepekimab, hFc Tag with the EC50 of 38.5ng/ml for Human IL-33<sup>red</sup> and not bind Human IL-33<sup>ox</sup> determined by ELISA. Immobilized Human IL-33, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Itepekimab, hFc Tag with the EC50 of 38.5ng/ml for Human IL-33red and not bind Human IL-33ox determined by ELISA.
Human IL-33<sup>ox</sup> on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-33ox on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-33<sup>ox</sup> is greater than 90% as determined by SEC-HPLC. The purity of Human IL-33ox is greater than 90% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...