• Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human P-Selectin, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).
  • Amino Acid Range: Trp42-Ala771
Catalog #SEL-HM10P
Price
Regular price $24750
Regular price Sale price $24750
/
Size
Avantor VWR Fisher Scientific

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Human P-Selectin/CD62P Protein (SEL-HM10P) Summary

Product Description

 Recombinant Human P-Selectin/CD62P Protein is expressed from HEK293 with His tag at the C-terminus.It contains Trp42-Ala771.

Product Background

 Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation.

Human P-Selectin/CD62P Protein (SEL-HM10P) Specifications/Details

Protein
 P-Selectin/CD62P
Synonyms
 FLJ45155; GMP140; GRMP; PADGEM; PSEL; P-Selectin; SELP; CD62P; CD62; LECAM3
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Trp42-Ala771
Molecular Weight
 The protein has a predicted MW of 81.05 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human P-Selectin, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).
Product Tag
 C-His
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human P-Selectin, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test). Immobilized Human P-Selectin, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA (QC Test).
Human P-Selectin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human P-Selectin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human P-Selectin is greater than 95% as determined by SEC-HPLC. The purity of Human P-Selectin is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...