Human P-Selectin/CD62P Protein (SEL-HM10P)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human P-Selectin, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Trp42-Ala771

Catalog: SEL-HM10P

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Product Description	Recombinant Human P-Selectin/CD62P Protein is expressed from HEK293 with His tag at the C-terminus.It contains Trp42-Ala771.
Product Background	Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation.
Product Category	Recombinant Protein

Target	P-Selectin/CD62P
Gene/Protein ID	P16109
Species	Human
Exact Sequence	Trp42-Ala771
Molecular Weight	The protein has a predicted MW of 81.05 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human P-Selectin, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human P-Selectin, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
	The purity of Human P-Selectin is greater than 95% as determined by SEC-HPLC.
	Human P-Selectin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.