Human PAH (D415N) Protein (PAH-HB001)
Human PAH (D415N) Protein (PAH-HB001)
  • Express System: Baculovirus-Insect Cells(Sf9)
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Met1-Lys452 (D415N)
Catalog #PAH-HB001
Regular price $330.00
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Product Description

Recombinant Human PAH (D415N) Protein is expressed from Baculovirus-Insect Cells(Sf9) with His tag at the N-terminus.It contains Met1-Lys452 (D415N).

Product Background

Phenylalanine hydroxylase (PAH) is a member of aromatic amino acid hydroxylase (AAAHs) family, and catalyze phenylalanine (Phe) into tyrosine (Tyr). PAH is also an allosteric enzyme that maintains phenylalanine (Phe) below neurotoxic levels; its failure results in phenylketonuria, an inborn error of amino acid metabolism.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

PAH (D415N)

Synonyms

PH; PKU; PKU1;

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Met1-Lys452 (D415N)

Molecular Weight

The protein has a predicted MW of 52.82 kDa same as Bis-Tris PAGE result.

Product Tag

N-His

Expression System

Baculovirus-Insect Cells(Sf9)

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 20mM Tris, 0.5M NaCl (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human PAH (D415N) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human PAH (D415N) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human PAH (D415N) is greater than 95% as determined by SEC-HPLC. The purity of Human PAH (D415N) is greater than 95% as determined by SEC-HPLC.

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