Product Description |
Recombinant Human PAH (D415N) Protein is expressed from Baculovirus-Insect Cells with His tag at the N-terminus.It contains Met1-Lys452 (D415N). |
Product Background |
Phenylalanine hydroxylase (PAH) is a member of aromatic amino acid hydroxylase (AAAHs) family, and catalyze phenylalanine (Phe) into tyrosine (Tyr). PAH is also an allosteric enzyme that maintains phenylalanine (Phe) below neurotoxic levels; its failure results in phenylketonuria, an inborn error of amino acid metabolism. |
Product Category |
Recombinant Protein |
Target |
PAH (D415N) |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met1-Lys452 (D415N) |
Molecular Weight |
The protein has a predicted MW of 52.82 kDa same as Bis-Tris PAGE result. |
Product Tag |
N-His |
Express System |
Baculovirus-Insect Cells |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20mM Tris, 0.5M NaCl (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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