Human PAH (D415N) Protein (PAH-HB001)
Human PAH (D415N) Protein (PAH-HB001)
  • Express System: Baculovirus-Insect Cells(Sf9)
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Amino Acid Range: Met1-Lys452 (D415N)
Catalog #PAH-HB001
Regular price $300.00
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Product Description

Recombinant Human PAH (D415N) Protein is expressed from Baculovirus-Insect Cells(Sf9) with His tag at the N-terminus.It contains Met1-Lys452 (D415N).

Product Background

Phenylalanine hydroxylase (PAH) is a member of aromatic amino acid hydroxylase (AAAHs) family, and catalyze phenylalanine (Phe) into tyrosine (Tyr). PAH is also an allosteric enzyme that maintains phenylalanine (Phe) below neurotoxic levels; its failure results in phenylketonuria, an inborn error of amino acid metabolism.

Product Category

Recombinant Protein / Antibody discovery

Protein

PAH (D415N)

Synonyms

PH; PKU; PKU1;

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Met1-Lys452 (D415N)

Molecular Weight

The protein has a predicted MW of 52.82 kDa same as Bis-Tris PAGE result.

Product Tag

N-His

Expression System

Baculovirus-Insect Cells(Sf9)

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 20mM Tris, 0.5M NaCl (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human PAH (D415N) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human PAH (D415N) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human PAH (D415N) is greater than 95% as determined by SEC-HPLC. The purity of Human PAH (D415N) is greater than 95% as determined by SEC-HPLC.

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