SDS-PAGE gel image showing molecular weight ladder and a clear single band in the sample lane, indicating expression and purity of the target protein.
  • Express System: Baculovirus-Insect Cells(Sf9)
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Amino Acid Range: Met1-Val322
Catalog #PBK-HB101
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Size
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Human PBK/TOPK Protein (PBK-HB101) Summary

Product Description

 Recombinant Human PBK/TOPK Protein is expressed from Baculovirus-Insect Cells(Sf9) with His tag at the C-terminus.It contains Met1-Val322.

Product Background

 T-lymphokine-activated killer cell-originated protein kinase (TOPK, also known as PDZ-binding kinase or PBK) plays a crucial role in cell cycle regulation and mitotic progression. Abnormal overexpression or activation of TOPK has been observed in many cancers, including colorectal cancer, triple-negative breast cancer, and melanoma, and it is associated with increased development, dissemination, and poor clinical outcomes and prognosis in cancer. Moreover, TOPK phosphorylates p38, JNK, ERK, and AKT, which are involved in many cellular functions, and participates in the activation of multiple signaling pathways related to MAPK, PI3K/PTEN/AKT, and NOTCH1.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human PBK/TOPK Protein (PBK-HB101) Specifications/Details

Protein
 PBK/TOPK
Synonyms
 CT84; Nori-3; Nori3; SPK; TOPK; PBK
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Met1-Val322
Molecular Weight
 The protein has a predicted MW of 37.61 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result.
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 Baculovirus-Insect Cells(Sf9)
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human PBK on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human PBK on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Other Available Proteins:
Finding available proteins...
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support@kactusbio.us

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