Product Description |
Recombinant Human PBK/TOPK Protein is expressed from Baculovirus-Insect Cells with His tag at the C-terminus.It contains Met1-Val322. |
Product Background |
T-lymphokine-activated killer cell-originated protein kinase (TOPK, also known as PDZ-binding kinase or PBK) plays a crucial role in cell cycle regulation and mitotic progression. Abnormal overexpression or activation of TOPK has been observed in many cancers, including colorectal cancer, triple-negative breast cancer, and melanoma, and it is associated with increased development, dissemination, and poor clinical outcomes and prognosis in cancer. Moreover, TOPK phosphorylates p38, JNK, ERK, and AKT, which are involved in many cellular functions, and participates in the activation of multiple signaling pathways related to MAPK, PI3K/PTEN/AKT, and NOTCH1. |
Product Category |
Recombinant Protein |
Target |
PBK/TOPK |
Synonyms |
CT84; Nori-3; Nori3; SPK; TOPK; PBK |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met1-Val322 |
Molecular Weight |
The protein has a predicted MW of 37.61 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Express System |
Baculovirus-Insect Cells |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human PBK on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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