Human PBK/TOPK Protein (PBK-HB101)

  • Express System: Baculovirus-Insect Cells
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Exact Sequence: Met1-Val322

Catalog: PBK-HB101

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Product Description Recombinant Human PBK/TOPK Protein is expressed from Baculovirus-Insect Cells with His tag at the C-terminus.It contains Met1-Val322.

Product Background

 T-lymphokine-activated killer cell-originated protein kinase (TOPK, also known as PDZ-binding kinase or PBK) plays a crucial role in cell cycle regulation and mitotic progression. Abnormal overexpression or activation of TOPK has been observed in many cancers, including colorectal cancer, triple-negative breast cancer, and melanoma, and it is associated with increased development, dissemination, and poor clinical outcomes and prognosis in cancer. Moreover, TOPK phosphorylates p38, JNK, ERK, and AKT, which are involved in many cellular functions, and participates in the activation of multiple signaling pathways related to MAPK, PI3K/PTEN/AKT, and NOTCH1.

Product Category

 Recombinant Protein

Target

 PBK/TOPK

Gene/Protein ID

 Q96KB5-1

Species

 Human

Exact Sequence

 Met1-Val322
Molecular Weight The protein has a predicted MW of 37.61 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Tris-Bis PAGE result.

Product Tag

 C-His

Express System

 Baculovirus-Insect Cells
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human PBK on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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