Human PCSK9 Protein (PCS-HM190)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human PCSK9, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PCSK9 antibody, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA. The affinity constant of 0.35 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln31-Gln692

Catalog: PCS-HM190

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Product Description Recombinant Human PCSK9 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Gln31-Gln692.
Product Category Recombinant Protein
Target PCSK9
Synonyms PC9; PCSK9; FH3; HCHOLA3; LDLCQ1; NARC-1
Gene/Protein ID Q8NBP7-1
Species Human
Exact Sequence Gln31-Gln692
Molecular Weight Due to autocatalytic cleavage, the protein release the pro-form (59 kDa) and mature form (14 kDa). Due to glycosylation, the protein migrates to 65-68 kDa (pro-form) and 15kDa (mature form) based on Tris-Bis PAGE result.
Activity Immobilized Human PCSK9, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PCSK9 antibody, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA. The affinity constant of 0.35 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Liquid
Shipping Shipped with blue ice.
Formulation Supplied as 0.22μm filtered solution in 20mM PB, 300mM NaCl, 10% glycerol (pH 7.4). Please dilute to the desired concentration according to the concentration of the solution shown on the product label.
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
Human LDLR, His Tag immobilized on CM5 Chip can bind Human PCSK9, His Tag with an affinity constant of 0.35 nM as determined in SPR assay (Biacore T200).
Human VLDLR, His Tag immobilized on CM5 Chip can bind Human PCSK9, His Tag with an affinity constant of 9.78 μM as determined in SPR assay (Biacore T200).
Immobilized Human PCSK9, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PCSK9 antibody, hFc Tag with the EC50 of 0.16μg/ml determined by ELISA.
The purity of Human PCSK9 is greater than 95% as determined by SEC-HPLC.
Human PCSK9 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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