Human PD-1/PDCD1 Protein (PD1-HM301)

Express System: HEK293
Product Tag: C-mFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PD-1, mFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 68ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Leu25-Gln167

Catalog: PD1-HM301

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Product Description	Recombinant Human PD-1/PDCD1 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Leu25-Gln167.
Product Background	Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors.PD1 is a inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation.
Product Category	Recombinant Protein

Target	PD-1/PDCD1
Synonyms	PDCD1; PD1; CD279; SLEB2; PD-1
Gene/Protein ID	Q15116-1
Species	Human
Exact Sequence	Leu25-Gln167
Molecular Weight	The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 64-68 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human PD-1, mFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 68ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-mFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human PD-1, mFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 68ng/ml determined by ELISA.
	Immobilized Human PD-1, mFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA (QC Test).
	The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC.
	Human PD-1 on Tris-Bis PAGE under reduced condition.The purity is greater than 95%.