Human PD-1/PDCD1 Protein (PD1-HM301)

  • Express System: Expi293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human PD-1, mFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 68ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu25-Gln167

Catalog: PD1-HM301

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Product Description Recombinant Human PD-1/PDCD1 Protein is expressed from Expi293 with mFc at the C-terminal.; It contains Leu25-Gln167.
Product Category Recombinant Protein
Target PD-1/PDCD1
Synonyms PDCD1; PD1; CD279; SLEB2; PD-1
Gene/Protein ID Q15116-1
Species Human
Exact Sequence Leu25-Gln167
Molecular Weight The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 64-68 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human PD-1, mFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 68ng/ml determined by ELISA. See testing image for detail.
Product Tag C-mFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human PD-1, mFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 68ng/ml determined by ELISA.
Immobilized Human PD-1, mFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.22μg/ml determined by ELISA.
The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC.
Human PD-1 on Tris-Bis PAGE under reduced condition.The purity is greater than 95%.

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