Human PD-1/PDCD1 Protein (PD1-HM301)
Human PD-1/PDCD1 Protein (PD1-HM301)
Human PD-1/PDCD1 Protein (PD1-HM301)
Human PD-1/PDCD1 Protein (PD1-HM301)
  • Express System: HEK293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human PD-1, mFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 68ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu25-Gln167
Catalog #PD1-HM301
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Product Description
 Recombinant Human PD-1/PDCD1 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Leu25-Gln167.
Product Background
 Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors.PD1 is a inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation.
Product Category
 Recombinant Protein
Target
 PD-1/PDCD1
Synonyms
 PDCD1; PD1; CD279; SLEB2; PD-1
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu25-Gln167
Molecular Weight
 The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 64-68 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human PD-1, mFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 68ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-mFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human PD-1, mFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 68ng/ml determined by ELISA.
Immobilized Human PD-1, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA (QC Test).
Human PD-1 on Bis-Tris PAGE under reduced condition.The purity is greater than 95%.
The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC.
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