Product Description |
Recombinant Human PD-1/PDCD1 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus.It contains Leu25-Gln167. |
Product Background |
Programmed Death-1 receptor (PD-1), also known as CD279, is type I transmembrane protein belonging to the CD28 family of immune regulatory receptors.PD1 is a inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self. Delivers inhibitory signals upon binding to ligands CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:21276005). Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation. |
Product Category |
Recombinant Protein |
Target |
PD-1/PDCD1 |
Synonyms |
PDCD1; PD1; CD279; SLEB2; PD-1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu25-Gln167 |
Molecular Weight |
The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 64-68 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human PD-1, mFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 68ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-mFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human PD-1, mFc Tag at 0.5ug/ml (100ul/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 68ng/ml determined by ELISA. |
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Immobilized Human PD-1, mFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.11ug/ml determined by ELISA (QC Test). |
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Human PD-1 on Bis-Tris PAGE under reduced condition.The purity is greater than 95%. |
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The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC. |
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