Human PD-1/PDCD1 Protein (PD1-HM301)

  • Express System: Expi293
  • Product Tag: C-mFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human PD-1, mFc Tag at 0.5_g/ml (100_l/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 68ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu25-Gln167

Catalog: PD1-HM301

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Product Description Recombinant Human PD-1/PDCD1 Protein is expressed from Expi293 with mFc at the C-terminal.It contains Leu25-Gln167.
Product Category Recombinant Protein
Target PD-1/PDCD1
Synonyms PDCD1; PD1; CD279; SLEB2; PD-1
Gene/Protein ID Q15116-1
Species Human
Exact Sequence Leu25-Gln167
Molecular Weight The protein has a predicted MW of 41.6 kDa. Due to glycosylation, the protein migrates to 64-68 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human PD-1, mFc Tag at 0.5_g/ml (100_l/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 68ng/ml determined by ELISA. See testing image for detail.
Product Tag C-mFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per _g by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22_m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 _g/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80¡C for 12 months, 4¡C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human PD-1, mFc Tag at 0.5_g/ml (100_l/Well). Dose response curve for Biotinylated Anti-PD-1 Ab., hFc Tag with the EC50 of 68ng/ml determined by ELISA.
Immobilized Human PD-1, mFc Tag at 1_g/ml (100_l/well) on the plate. Dose response curve for Human PD-L1, hFc Tag with the EC50 of 0.15_g/ml determined by ELISA.
The purity of Human PD-1 is greater than 95% as determined by SEC-HPLC.
Human PD-1 on Tris-Bis PAGE under reduced condition.The purity is greater than 95%.

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